GSK3 磷酸化并调节绿色革命蛋白 Rht-B1b，从而降低小麦株高。
GSK3 phosphorylates and regulates the Green Revolution protein Rht-B1b to reduce plant height in wheat.
机构信息
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
出版信息
Plant Cell. 2023 May 29;35(6):1970-1983. doi: 10.1093/plcell/koad090.
Abstract
The utilization of stabilized DELLA proteins Rht-B1b and Rht-D1b was crucial for increasing wheat (Triticum aestivum) productivity during the Green Revolution. However, the underlying mechanisms remain to be clarified. Here, we cloned a gain-of-function allele of the GSK3/SHAGGY-like kinase-encoding gene GSK3 by characterizing a dwarf wheat mutant. Furthermore, we determined that GSK3 interacts with and phosphorylates the Green Revolution protein Rht-B1b to promote it to reduce plant height in wheat. Specifically, phosphorylation by GSK3 may enhance the activity and stability of Rht-B1b, allowing it to inhibit the activities of its target transcription factors. Taken together, we reveal a positive regulatory mechanism for the Green Revolution protein Rht-B1b by GSK3, which might have contributed to the Green Revolution in wheat.
摘要
在绿色革命期间，稳定的 DELLA 蛋白 Rht-B1b 和 Rht-D1b 的利用对于提高小麦（Triticum aestivum）的生产力至关重要。然而，其潜在机制仍需阐明。在这里，我们通过对一个矮秆小麦突变体进行特征描述，克隆了一个编码 GSK3/SHAGGY 样激酶的基因 GSK3 的功能获得性等位基因。此外，我们确定 GSK3 与绿色革命蛋白 Rht-B1b 相互作用并使其磷酸化，以促进其降低小麦株高。具体而言，GSK3 的磷酸化可能增强 Rht-B1b 的活性和稳定性，使其能够抑制其靶转录因子的活性。总之，我们揭示了 GSK3 对绿色革命蛋白 Rht-B1b 的正向调控机制，这可能为小麦的绿色革命做出了贡献。
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