Wu Zhongnan, Li Chong, Li Junhao, Wang Tanggan, Li Meifeng, Zhao Leyi, Ye Huimei, Chen Jiaheng, Zan Jiajia, Song Lijun, Zhang Qian, Zhang Shaojie
The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China.
College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
Ultrason Sonochem. 2025 Jan;112:107206. doi: 10.1016/j.ultsonch.2024.107206. Epub 2024 Dec 16.
Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol's environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28 % extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48-174.64 kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.
三相分配法（TPP）在分离生物活性多糖方面具有潜力，但叔丁醇对环境的影响限制了其应用。深共晶溶剂可作为叔丁醇的绿色可回收替代品。本研究引入了一种超声辅助深共晶溶剂三相分配法（UA-TPP-DES）系统来提取和纯化西洋参多糖（AGP）。由月桂酸和壬酸组成（摩尔比 = 1:1）的优化深共晶溶剂DES-4在特定条件下实现了35.28%的提取率，并且可以循环使用五次，产率损失最小。AGP-DES-4具有较宽的分子量分布（2.48 - 174.64 kDa），主要由甘露糖、葡萄糖、半乳糖和阿拉伯糖组成。体内果蝇模型表明，AGP-DES-4可提高患溃疡性结肠炎果蝇的存活率，并通过调节肠上皮细胞（IEC）和肠干细胞（ISC）的增殖与分化来增强肠道屏障功能。我们的结果突出了AGP-DES-4提取方法的有效性及其对治疗溃疡性结肠炎的潜在治疗价值。
