Xue Yongkang, Hu Zhengyu, Jiang Yuxin, Li Man, Liu Yanan, Duan Yuanqi, Zhou Wei, Sun Jinfeng, Li Gao
Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002 Jilin Province, China.
Ultrason Sonochem. 2025 Jun 27;120:107449. doi: 10.1016/j.ultsonch.2025.107449.
The objective of this study was to efficiently extract components with neuroprotective activity from the root of Paeonia lactiflora Pall. (RPL). The neuroprotective extracts of RPL were extracted by ultrasound-assisted deep eutectic solvents (DESs) method, and optimized by response surface methodology with PC12 cell viability as a guide. The optimal process: DESs (composed of L-Proline and Glycerol, Betaine monohydrate and Benzyl alcohol in a ratio of 7:3 (v/v) with 60 % water), extraction time 22 min, liquid-to-solid ratio 42 mL/g, temperature 51 °C, ultrasonic power 243 W. These conditions yielded extracts with cell viability 81.44 ± 0.18 % (n = 3), which is much higher than conventional heat and standard DESs extraction methods. Then, the RPL neuroprotective purified extract (RNPE) was obtained by AB-8 macroporous resin, and characterized by UPLC-Q-TOF-MS analysis. The results showed that RNPE was mainly composed of 50 compounds, including terpenoids, phenolics, sterols and so on, and it showed significant neuroprotection in middle cerebral artery occlusion (MCAO) mice. These results established ultrasound-assisted deep eutectic solvent extraction (UADE) as a sustainable approach for extracting neuroprotective agents from RPL, combining process efficiency with bioactive preservation. The integrated method, from screening of DESs to optimization of ultrasound-assisted extraction conditions and in vivo validation, establishes an efficient template for neuroprotective components.
本研究的目的是从芍药根中高效提取具有神经保护活性的成分。采用超声辅助深共熔溶剂（DESs）法提取芍药根的神经保护提取物，并以PC12细胞活力为指标，通过响应面法进行优化。最佳工艺为：DESs（由L-脯氨酸和甘油、一水甜菜碱和苯甲醇按7:3（v/v）比例与60%水组成），提取时间22分钟，液固比42 mL/g，温度51℃，超声功率243 W。在这些条件下得到的提取物细胞活力为81.44±0.18%（n = 3），远高于传统加热法和标准DESs提取法。然后，通过AB-8大孔树脂获得芍药根神经保护纯化提取物（RNPE），并通过超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）分析进行表征。结果表明，RNPE主要由50种化合物组成，包括萜类、酚类、甾醇等，并且在大脑中动脉闭塞（MCAO）小鼠中显示出显著的神经保护作用。这些结果确立了超声辅助深共熔溶剂萃取（UADE）作为从芍药根中提取神经保护剂的可持续方法，该方法兼具工艺效率和生物活性保存。从DESs筛选到超声辅助提取条件优化以及体内验证的综合方法，为神经保护成分建立了一个高效模板。
