Bian S, Han R, Xiao H, Yue J, Wang S T, Lin H Z, Ma L
Department of prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao266003, China School of Stomatology, Qingdao University, Qingdao266023, China.
Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao266003, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2024 Dec 6;58(12):1976-1986. doi: 10.3760/cma.j.cn112150-20240628-00522.
To analyze the effect of Iron deficiency anemia (IDA) and Black stain (BS) on the microbial community of dental plaque. A total of 136 preschool children aged 3 to 6 years old from 12 kindergartens in Shinan District and Shibei District of Qingdao City were investigated by using a cluster sampling method from April to May 2019. They were divided into two groups based on oral examination: the early childhood caries (ECC) group and the caries-free (CF) group. According to whether they had IDA and BS, they were further divided into four groups: the IDA with caries (IDA-ECC) group, the non-IDA with caries (NIDA-ECC) group, the BS without caries (BS-CF) group and the non-BS without caries (NBS-CF) group. The gingival plaque of the study subjects was collected. The 16S rRNA gene was sequenced by using Illumina MiSeq high-throughput sequencing technology. The composition, community structure and different bacteria genera of the microbial communities between the groups were compared and analyzed. Potential biomarkers within each group were further identified by linear discriminant analysis of effect size (LEfSe). The 136 children were aged (5.11±0.87) years old, with 80 boys (58.82%). There were statistically significant differences in the microbial composition, structure and function of oral plaque between the ECC and CF groups (<0.05). There were statistically significant differences in the microbial richness and diversity of oral plaque between the IDA-ECC and NIDA-ECC groups (<0.05). There was no significant difference in microbial diversity index between the BS-CF and NBS-CF groups (>0.05). The LEfSe analysis results showed that 41, 31 and 9 taxa with different relative abundance were identified between the ECC and CF groups, IDA-ECC and NIDA-ECC groups, and BS-CF and NBS-CF groups, respectively. IDA and BS have an effect on the microecological diversity and microbial community function of oral plaque in young children with early childhood caries.
分析缺铁性贫血（IDA）和黑斑（BS）对牙菌斑微生物群落的影响。2019年4月至5月，采用整群抽样方法，对青岛市市南区和市北区12所幼儿园的136名3至6岁学龄前儿童进行了调查。根据口腔检查将他们分为两组：幼儿龋（ECC）组和无龋（CF）组。根据是否患有IDA和BS，进一步将他们分为四组：患龋伴IDA（IDA-ECC）组、患龋不伴IDA（NIDA-ECC）组、无龋伴BS（BS-CF）组和无龋不伴BS（NBS-CF）组。收集研究对象的龈下菌斑。采用Illumina MiSeq高通量测序技术对16S rRNA基因进行测序。比较分析各组微生物群落的组成、群落结构及不同细菌属。通过效应大小线性判别分析（LEfSe）进一步鉴定每组中的潜在生物标志物。136名儿童年龄为（5.11±0.87）岁，其中男孩80名（58.82%）。ECC组和CF组口腔菌斑的微生物组成、结构和功能存在统计学差异（<0.05）。IDA-ECC组和NIDA-ECC组口腔菌斑的微生物丰富度和多样性存在统计学差异（<0.05）。BS-CF组和NBS-CF组的微生物多样性指数无显著差异（>0.05）。LEfSe分析结果显示，ECC组和CF组、IDA-ECC组和NIDA-ECC组、BS-CF组和NBS-CF组之间分别鉴定出41、31和9个相对丰度不同的分类单元。IDA和BS对患有幼儿龋的幼儿口腔菌斑的微生态多样性和微生物群落功能有影响。
