Retzmann Anika, Miller Kerri A, Mohamed Fwziah Ali Abdalali, Wieser Michael E
Department of Physics and Astronomy, University of Calgary, 2500 University Dr. NW, Calgary, AB, T2N 1N4, Canada.
Arnie Charbonneau Cancer Institute, University of Calgary, 3280 Hospital Dr. NW, Calgary, AB, T2N 4Z6, Canada.
Anal Bioanal Chem. 2025 Feb;417(4):835-846. doi: 10.1007/s00216-024-05702-1. Epub 2024 Dec 23.
A fully automated dual-column purification procedure for Zn from biological samples, designed for subsequent Zn isotopic analysis, is presented that utilizes the prepFAST MC™ system (Elemental Scientific), DGA resin (TrisKem International), and TK201 resin (TrisKem International). The procedure developed enables the unattended processing of 20 samples per day and is characterized by low and reproduceable blanks (< 1.5 ng), no carry-over or memory effect, high reusability (> 50 times), high Zn yields 100.1% ± 5.3% (2 SD, N = 22), and strong robustness to matrix variations across biological samples (bone, liver, hair, blood). Additionally, Zn isotopic analysis using MC-ICP-MS showed no significant on-column fractionation. The measured δZn/Zn values for NIST SRM 1400 (0.67‰ ± 0.07‰, U, k = 2), NIST SRM 1486 (0.91‰ ± 0.06‰, U, k = 2), NIST SRM 1577c (- 0.45‰ ± 0.05‰, U, k = 2), ERM-DB001 (- 0.35‰ ± 0.05‰, U, k = 2), GBW09101 (- 0.32‰ ± 0.08‰, U, k = 2), and SeroNorm whole blood L-3 (-0.15 ‰ ± 0.05 ‰, U, k = 2) are consistent with published values. The procedure developed makes Zn, an analytically challenging isotope system, more accessible, feasible, and reliable for a broader range of users while enabling high sample throughput.
本文介绍了一种用于生物样品中锌的全自动双柱纯化程序，该程序专为后续锌同位素分析而设计，采用了prepFAST MC™系统（Elemental Scientific公司）、DGA树脂（TrisKem International公司）和TK201树脂（TrisKem International公司）。所开发的程序能够实现每天无人值守处理20个样品，其特点是空白值低且可重复（<1.5 ng），无残留或记忆效应，可重复使用性高（>50次），锌回收率高，为100.1%±5.3%（2标准偏差，N = 22），并且对不同生物样品（骨骼、肝脏、毛发、血液）的基质变化具有很强的耐受性。此外，使用MC-ICP-MS进行的锌同位素分析显示柱上无明显分馏现象。对NIST SRM 1400（0.67‰±0.07‰，扩展不确定度，k = 2）、NIST SRM 1486（0.91‰±0.06‰，扩展不确定度，k = 2）、NIST SRM 1577c（-0.45‰±0.05‰，扩展不确定度，k = 2）、ERM-DB001（-0.35‰±0.05‰，扩展不确定度，k = 2）、GBW09101（-0.32‰±0.08‰，扩展不确定度，k = 2）和SeroNorm全血L-3（-0.15‰±0.05‰，扩展不确定度，k = 2）测量的δZn/Zn值与已发表的值一致。所开发的程序使锌这种分析具有挑战性的同位素系统对更广泛的用户来说更容易获得、可行且可靠，同时实现了高样品通量。
