Meaney M J, Aitken D H
Brain Res. 1985 Feb 25;328(1):176-80. doi: 10.1016/0006-8993(85)91340-x.
The work described in this paper presents evidence for the existence of specific glucocorticoid receptors in the rat frontal cortex. Using [3H]dexamethasone we found a Kd approximately 6 nM and a Bmax approximately 270 fmol/mg protein, concentrations that were about 75% of those found in hippocampus. [3H]dexamethasone binding in the frontal cortex, like that in hippocampus, was regulated by the corticosterone: thus, one-week treatment with corticosterone results in a decrease and long-term adrenalectomy results in an increase in [3H]dexamethasone binding. Developmentally, as reported for other brain regions, [3H]dexamethasone binding in frontal cortex was low during the first week of life and then rose during the following 10 days to approximate adult levels. These results are discussed in terms of providing a possible mechanism for the influence of corticoids on catecholamine activity in the frontal cortex.
本文所述的研究工作为大鼠额叶皮质中存在特异性糖皮质激素受体提供了证据。使用[3H]地塞米松，我们发现解离常数（Kd）约为6 nM，最大结合容量（Bmax）约为270 fmol/mg蛋白质，这些浓度约为海马体中所发现浓度的75%。额叶皮质中[3H]地塞米松的结合，与海马体中的情况一样，受皮质酮调节：因此，用皮质酮进行一周的治疗会导致[3H]地塞米松结合减少，而长期肾上腺切除术则会导致[3H]地塞米松结合增加。在发育过程中，如同其他脑区的报道一样，额叶皮质中[3H]地塞米松的结合在出生后的第一周较低，随后在接下来的10天内上升至接近成年水平。本文将根据这些结果讨论皮质激素对额叶皮质中儿茶酚胺活性产生影响的可能机制。
