Zhou Zhiming, Guo Zongfeng, Lu Xiaomin, Xu Xiaoqing
Nantong University, Affiliated Haian Hospital, Department of Interventional Oncology, Nantong, China.
Nantong University, Affiliated Haian Hospital, Department of Anesthesiology, Nantong, China.
J Med Biochem. 2024 Sep 6;43(5):664-670. doi: 10.5937/jomb0-48160.
This research aimed to observe the mechanism of lactate dehydrogenase (LDH) and interleukin 8 (IL8) in pancreatic cancer pain and their correlation with pain degree.
126 patients with pancreatic cancer who visited our hospital from January 2021 to February 2023 were selected. The patients were divided into groups of 58 patients with low pain (13 points) and 68 patients with high pain (410 points) by visual analog scale (VAS). And 50 health examinees in the same period were selected as the healthy control group. The serum LDH and IL-8 concentrations are analyzed by enzyme-linked immunosorbent assay, and the subjective pain grading method score is analyzed. The differences in LDH and IL-8 concentrations among the three groups of patients were compared. Pearson correlation analysis was used to investigate the correlation between LDH, IL-8 concentrations, and patient pain. Binary logistic regression was used to determine independent risk factors for high pain, and ROC curves were used to analyze the diagnostic efficacy of each indicator.
The serum LDH and IL-8 concentrations in the high-pain group were exceed the low-pain group's (P<0.05). The serum LDH and IL-8 concentrations in the low-pain group exceeded the healthy control group's (P<0.05). Pearson correlation analysis revealed a positive correlation between serum LDH concentration and pain grading (r=0.736, P=0.000). The serum IL-8 positively correlates with pain grading (r=0.680, P=0.000). Serum LDH and IL-8 concentrations positively correlate (r=0.589, P=0.000). LDH and IL-8 concentrations are independent risk factors for high pain levels (OR=1.033, 1.142, P<0.05). The logistic regression prediction model formula was used: Y=constant+B1X1+B2X2+...+BnXn to set the joint diagnostic prediction model as -12.063+ 0.033×LDH+0.133×IL-8. The areas under the ROC curves of LDH, IL-8, and predictive model (LDH+IL-8) in patients with high pain were 0.925, 0.945, and 0.974, respectively. The relevant standards for LDH are >190 U/L, IL-8 is >36 pg/mL, and the relevant standards for prediction models are >5.75.
LDH and IL-8 participate in the pain aggravation process of pancreatic cancer and are closely related to the pain grading. The combination of LDH and IL-8 can be used as a biological indicator to evaluate the pain severity of pancreatic cancer and provide a reference for clinical diagnosis and treatment.
本研究旨在观察乳酸脱氢酶（LDH）和白细胞介素8（IL8）在胰腺癌疼痛中的作用机制及其与疼痛程度的相关性。
选取2021年1月至2023年2月来我院就诊的126例胰腺癌患者。采用视觉模拟评分法（VAS）将患者分为低疼痛组（13分）58例和高疼痛组（410分）68例。同期选取50例健康体检者作为健康对照组。采用酶联免疫吸附测定法分析血清LDH和IL-8浓度，并分析主观疼痛分级法评分。比较三组患者LDH和IL-8浓度的差异。采用Pearson相关分析研究LDH、IL-8浓度与患者疼痛之间的相关性。采用二元逻辑回归确定高疼痛的独立危险因素，并采用ROC曲线分析各指标的诊断效能。
高疼痛组血清LDH和IL-8浓度高于低疼痛组（P<0.05）。低疼痛组血清LDH和IL-8浓度高于健康对照组（P<0.05）。Pearson相关分析显示血清LDH浓度与疼痛分级呈正相关（r=0.736，P=0.000）。血清IL-8与疼痛分级呈正相关（r=0.680，P=0.000）。血清LDH与IL-8浓度呈正相关（r=0.589，P=0.000）。LDH和IL-8浓度是高疼痛水平的独立危险因素（OR=1.033，1.142，P<0.05）。采用逻辑回归预测模型公式：Y=常数+B1X1+B2X2+...+BnXn，将联合诊断预测模型设定为-12.063+0.033×LDH+0.133×IL-8。高疼痛患者中LDH、IL-8及预测模型（LDH+IL-8）的ROC曲线下面积分别为0.925、0.945和0.974。LDH的相关标准为>190 U/L，IL-8为>36 pg/mL，预测模型的相关标准为>5.75。
LDH和IL-8参与胰腺癌疼痛加重过程，且与疼痛分级密切相关。LDH和IL-8联合可作为评估胰腺癌疼痛严重程度的生物学指标，为临床诊疗提供参考。
