Nazemi Salman Bahareh, Saburi Ehsan, Gheidari Mahtab Mohammadi, Farsadeghi Mahya, Basir Shabestari Samira
Dept. of Pediatrics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.
Dept. of Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Dent (Shiraz). 2024 Dec 1;25(4):342-348. doi: 10.30476/dentjods.2023.99173.2131. eCollection 2024 Dec.
Success of pulpotomy of primary teeth depends on biological and cytotoxic effects of pulp capping agents. Mineral trioxide aggregate (MTA), Biodentine, calcium enriched mixture (CEM) cement, and ferric sulfate (FS) are among the commonly used pulp capping agents (PCAs) for pulpotomy, and their successful application has been previously evaluated.
This study aimed to compare the cytotoxicity of PCAs against mesenchymal stem cells isolated from human exfoliated deciduous teeth (SHEDs).
In this study, SHEDs were exposed to MTA, Biodentine, CEM cement, and FS for 24 and 72 hours. The methyl thiazolyl tetrazolium (MTT) assay was performed for five different concentrations of PCAs after 24 and 72 hours of exposure. Data were analyzed by ANOVA.
Generally, the biocompatibility increased by reduction in concentration. All tested concentrations showed higher biocompatibility at 72 hours compared with 24 hours (< 0.0001). Comparison of cytotoxicity of different biomaterials revealed no significant difference at any time point (> 0.05).
In general, the cytotoxicity of MTA, Biodentine, CEM cement, and FS was comparable, with no significant difference. Cytotoxicity decreased over time and by a reduction in concentration of biomaterials. MTA and Biodentine showed maximum biocompatibility followed by FS, and CEM cement.
乳牙活髓切断术的成功取决于盖髓剂的生物学和细胞毒性作用。三氧化矿物凝聚体（MTA）、生物活性玻璃陶瓷（Biodentine）、富钙混合物（CEM）粘固剂和硫酸铁（FS）是活髓切断术中常用的盖髓剂（PCA），此前已对它们的成功应用进行了评估。
本研究旨在比较PCA对从人脱落乳牙（SHEDs）分离的间充质干细胞的细胞毒性。
在本研究中，将SHEDs分别暴露于MTA、Biodentine、CEM粘固剂和FS中24小时和72小时。在暴露24小时和72小时后，对五种不同浓度的PCA进行甲基噻唑基四氮唑（MTT）测定。数据采用方差分析进行分析。
一般来说，生物相容性随浓度降低而增加。与24小时相比，所有测试浓度在72小时时均显示出更高的生物相容性（<0.0001）。不同生物材料细胞毒性的比较在任何时间点均无显著差异（>0.05）。
总体而言，MTA、Biodentine、CEM粘固剂和FS的细胞毒性相当，无显著差异。细胞毒性随时间和生物材料浓度的降低而降低。MTA和Biodentine表现出最大的生物相容性，其次是FS和CEM粘固剂。
