Dou Lei, Yan Qifang, Yang Deqin
Department of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, P.R. China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, P.R. China.
Exp Ther Med. 2020 Mar;19(3):2377-2383. doi: 10.3892/etm.2020.8444. Epub 2020 Jan 10.
The aim of the present study was to investigate the effect of calcium hydroxide [Ca(OH)], mineral trioxide aggregate (MTA), iRoot BP, platelet-rich fibrin (PRF) and concentrated growth factors (CGF) on the proliferation, viability, apoptosis and mineralization of human dental pulp cells (HDPCs). HDPCs were treated with Ca(OH), MTA, iRoot BP, PRF and CGF exudates. Cell viability, apoptosis, proliferation, cell cycle and alkaline phosphatase (ALP) activity were evaluated . PRF significantly increased the cell proliferation as compared with that in the MTA and iRoot BP groups on day 3. The CGF group displayed higher proliferation rates as compared with that in the MTA group on days 3 and 7. The MTA group displayed the highest ALP activity on days 1 and 3, and the CGF group on day 7. Ca(OH) inhibited cell proliferation and the percentages of dead and apoptotic cells were relatively higher in the Ca(OH) group on days 1, 3 and 7 compared with those in the other groups. In conclusion, PRF and CGF may be potential pulp-capping materials for vital pulp therapy. Future studies are required to confirm this.
本研究的目的是探讨氢氧化钙[Ca(OH)]、三氧化矿物凝聚体（MTA）、iRoot BP、富血小板纤维蛋白（PRF）和浓缩生长因子（CGF）对人牙髓细胞（HDPCs）增殖、活力、凋亡和矿化的影响。用Ca(OH)、MTA、iRoot BP、PRF和CGF渗出液处理HDPCs。评估细胞活力、凋亡、增殖、细胞周期和碱性磷酸酶（ALP）活性。与第3天MTA组和iRoot BP组相比，PRF显著增加细胞增殖。与第3天和第7天MTA组相比，CGF组显示出更高的增殖率。MTA组在第1天和第3天显示出最高的ALP活性，CGF组在第7天显示出最高的ALP活性。Ca(OH)抑制细胞增殖，与其他组相比，Ca(OH)组在第1天、第3天和第7天死亡和凋亡细胞的百分比相对较高。总之，PRF和CGF可能是活髓治疗中潜在的盖髓材料。需要进一步的研究来证实这一点。
