Li Yong, Qian Yisong, Lei Tianhua, Monaghan-Nichols Paula, Fu Mingui
bioRxiv. 2024 Dec 9:2024.12.09.627596. doi: 10.1101/2024.12.09.627596.
The current detection methods for glycoRNAs include metabolic labeling of living cells or animals and rPAL that directly detects native glycoRNAs by periodate oxidation and aldehyde ligation. Both of them have some limitations. Here we reported a simple and rapid detection of native glycoRNAs by using lectins. The method involves in several simple procedures: isolation of total RNA, northern blotting and detection with lectins. The advantages of this method include high sensitivity, very simple procedures and broader applications. We used this method detecting glycoRNA expression in the RNA samples from different species. We also detect the glycoRNA expression across the varieties of mouse and human tissues and compared with other detection methods. We are in the first to detect free glycoRNAs in the varieties of human biofluids. Overall, this simple and rapid method will provide a new tool for study of glycoRNA biology and clinical diagnosis in future.
目前用于检测糖基化RNA的方法包括对活细胞或动物进行代谢标记以及通过高碘酸盐氧化和醛连接直接检测天然糖基化RNA的rPAL。这两种方法都有一些局限性。在此，我们报道了一种使用凝集素简单快速检测天然糖基化RNA的方法。该方法涉及几个简单步骤：总RNA的分离、Northern印迹和凝集素检测。该方法的优点包括高灵敏度、非常简单的步骤和更广泛的应用。我们使用这种方法检测来自不同物种的RNA样本中的糖基化RNA表达。我们还检测了小鼠和人类各种组织中的糖基化RNA表达，并与其他检测方法进行了比较。我们首次在多种人类生物流体中检测到游离糖基化RNA。总体而言，这种简单快速的方法将为未来糖基化RNA生物学研究和临床诊断提供一种新工具。
