Goh K O
Cancer Genet Cytogenet. 1985 Mar 15;16(2):103-7. doi: 10.1016/0165-4608(85)90002-0.
Peripheral blood lymphocytes from five chronic lymphocytic leukemia (CLL) patients were cultured with PHA for 3-6 days. Chromosomal analysis with G-banding showed 25% of the diploid metaphases were pseudodiploid as a result of either a chromosomal deletion or a translocation. Abnormal clones with 18p- were seen in two patients, and two other patients had 18p- metaphases, but with other inconsistent abnormal chromosomes. None of the patients had trisomy 12, which has been thought by some to carry a poor prognosis. Although no conclusion can be reached regarding the significance of the chromosomal abnormalities in CLL, the type of chromosomal abnormalities in these patients suggests that they are the result of chromosomal breakage and abnormal repair.
从五名慢性淋巴细胞白血病（CLL）患者采集的外周血淋巴细胞用植物血凝素（PHA）培养3至6天。采用G显带技术进行染色体分析，结果显示25%的二倍体中期细胞由于染色体缺失或易位而呈假二倍体。两名患者出现了伴有18号染色体短臂缺失（18p-）的异常克隆，另外两名患者有18p-中期细胞，但伴有其他不一致的异常染色体。所有患者均未出现12号染色体三体，有些人认为12号染色体三体预后不良。虽然关于CLL中染色体异常的意义无法得出结论，但这些患者的染色体异常类型表明它们是染色体断裂和异常修复的结果。
