Jiang Yuhan, Li Tenghai, Liu Bingzhang, Tian Yufeng, Wang Yixin, Li Tian, Zhang Duo
Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, Changchun 130021, P. R. China.
ACS Omega. 2024 Dec 4;9(50):49597-49608. doi: 10.1021/acsomega.4c07489. eCollection 2024 Dec 17.
The main causes of failure for cartilage tissue engineering implants are tissue integration, inflammation, and infection. The development of biomaterials with antiforeign body response (FBR) is of particular importance. Herein, we developed a hydrogel loaded with anti-inflammatory drugs to reduce the inflammatory response that follows implantation. The human chondrocytes were used for in vitro study, and cell-laden hydrogel samples were implanted with the backs of rabbits for in vivo study. Soyasaponin Bb (SsBb) as a traditional Chinese medicine could significantly ( < 0.05) downregulate the expression levels of inflammation-related markers including iNOS, COX-2, and IL-6 in chondrocytes induced by IL-1β through the NF-κB signaling pathway. The in vitro experiments demonstrated that a gelatin-methacryloyl (GelMA) hydrogel loaded with SsBb (SsBb/GelMA) could similarly reduce the gene and protein expression levels of inflammation-related markers (iNOS, COX-2, and IL-6). The in vivo anti-inflammatory effects of the SsBb/GelMA hydrogels were assessed by immunohistochemical staining. The results demonstrated that SsBb/GelMA hydrogels inhibited the inflammatory response and downregulated the expression of the inflammatory cytokine IL-6. Therefore, SsBb/GelMA hydrogels are promising candidates for promoting anti-inflammation and cartilage tissue regeneration of implant surfaces.
软骨组织工程植入物失败的主要原因是组织整合、炎症和感染。开发具有抗异物反应（FBR）的生物材料尤为重要。在此，我们开发了一种负载抗炎药物的水凝胶，以减少植入后随之而来的炎症反应。使用人软骨细胞进行体外研究，并将负载细胞的水凝胶样品植入兔背部进行体内研究。大豆皂苷Bb（SsBb）作为一种中药，可通过NF-κB信号通路显著（<0.05）下调IL-1β诱导的软骨细胞中包括iNOS、COX-2和IL-6在内的炎症相关标志物的表达水平。体外实验表明，负载SsBb的明胶-甲基丙烯酰基（GelMA）水凝胶（SsBb/GelMA）同样可以降低炎症相关标志物（iNOS、COX-2和IL-6）的基因和蛋白表达水平。通过免疫组织化学染色评估SsBb/GelMA水凝胶的体内抗炎作用。结果表明，SsBb/GelMA水凝胶抑制了炎症反应，并下调了炎性细胞因子IL-6的表达。因此，SsBb/GelMA水凝胶有望成为促进植入物表面抗炎和软骨组织再生的候选材料。
