Ludwig Latasha, Vanderboon Emma N, Treleaven Heather, Wood R Darren, Schott Courtney R, Wood Geoffrey A
Department of Pathobiology, University of Guelph, Guelph N1G 2W1, Canada.
Biol Open. 2024 Dec 15;13(12). doi: 10.1242/bio.060483. Epub 2024 Dec 23.
MicroRNAs (miRNAs) are small non-coding RNA molecules that are present in all cell types and bodily fluids and are commonly dysregulated in cancer. miRNAs in cancer have been studied by measuring levels in cell lines, tumour tissues, and in circulation; however, no study has specifically investigated miRNA expression in patient-matched samples across all three sample types. Canine osteosarcoma is a well-established spontaneously occurring model of human osteosarcoma for which matched samples are available. We analysed a panel of miRNAs by real-time quantitative PCR and compared across patients and sample types. While some miRNAs are highly expressed in all three sample types, tumour tissue and cell lines had the most in common. There were several miRNAs that were highly expressed in plasma and tumour tissue but not in cell lines and likely represent miRNAs produced in the tumour microenvironment. Two highly expressed miRNAs were exclusive to plasma and are known to be expressed in circulating cells. This study highlights the importance of considering sample type when studying miRNAs in cancer and demonstrates the power of using patient-matched samples.
微小RNA（miRNA）是存在于所有细胞类型和体液中的小型非编码RNA分子，在癌症中通常表达失调。癌症中的miRNA已通过测量细胞系、肿瘤组织和循环系统中的水平进行研究；然而，尚无研究专门调查过患者匹配的所有三种样本类型中的miRNA表达情况。犬骨肉瘤是一种成熟的人类骨肉瘤自发模型，可获得匹配样本。我们通过实时定量PCR分析了一组miRNA，并在患者和样本类型之间进行了比较。虽然一些miRNA在所有三种样本类型中均高度表达，但肿瘤组织和细胞系的共同点最多。有几种miRNA在血浆和肿瘤组织中高度表达，但在细胞系中不表达，可能代表肿瘤微环境中产生的miRNA。两种高度表达的miRNA仅存在于血浆中，已知在循环细胞中表达。这项研究强调了在癌症研究中考虑样本类型的重要性，并证明了使用患者匹配样本的作用。
