Padva Leo, Gullick Jemma, Coe Laura J, Hansen Mathias H, De Voss James J, Crüsemann Max, Cryle Max J
Institute of Pharmaceutical Biology, University of Bonn, 53115, Bonn, Germany.
Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Chembiochem. 2025 Apr 1;26(7):e202400916. doi: 10.1002/cbic.202400916. Epub 2025 Jan 29.
The biarylitides are a recently discovered class of RiPP natural products that are fascinating both from the small size of the core peptides as well as the diversity of peptide crosslinking exhibited by the cytochrome P450 enzymes found in these systems. In this review, we address the discovery and biosynthetic diversity of these systems and discuss the methods and challenges of analysing the structures of these constrained cyclic peptides. We also discuss the structures of the P450 enzymes involved in these pathways and address the potential for alternate catalytic outcomes and activities as seen most recently with the inclusion of biarylitide related enzymes within rufomycin biosynthesis.
联芳基肽是最近发现的一类核糖体合成和翻译后修饰的天然产物，它们不仅因其核心肽的小尺寸而引人入胜，而且因其系统中发现的细胞色素P450酶所表现出的肽交联多样性而备受关注。在这篇综述中，我们阐述了这些系统的发现和生物合成多样性，并讨论了分析这些受限环肽结构的方法和挑战。我们还讨论了参与这些途径的P450酶的结构，并探讨了替代催化结果和活性的可能性，最近在鲁佛霉素生物合成中纳入与联芳基肽相关的酶时就观察到了这种情况。
