Dohadwala M, Ray P K
Cancer Chemother Pharmacol. 1985;14(2):135-8. doi: 10.1007/BF00434352.
At a high dose, cyclophosphamide (Cy, 200 mg/kg) causes depression of the enzyme activity of the hepatic mixed function oxygenase (MFO) system in Sprague-Dawley rats. The present report provides evidence for the early regeneration of the depleted enzyme activity in Cy-treated rats by purified protein A (P) of Staphylococcus aureus. Enzymes of the MFO system, such as aminopyrine demethylase and aryl hydrocarbon hydroxylase, were assayed and the content of cytochrome P-450 was determined. Inoculation of P (60 micrograms/kg) prior to Cy inoculation provides a better effect than P administration after Cy. The exact mechanism of P action is unknown. P-treated animals appear to have an ability to repair the damage caused by the toxic metabolites of Cy earlier than those in the Cy group. This property of protein A may become useful in accelerated regeneration of the enzyme activity in the hepatic MFO system following the toxic insult of Cy metabolites.
高剂量的环磷酰胺（Cy，200毫克/千克）会导致斯普拉格-道利大鼠肝脏混合功能氧化酶（MFO）系统的酶活性降低。本报告提供了证据，表明金黄色葡萄球菌的纯化蛋白A（P）可使经Cy处理的大鼠中耗尽的酶活性早期再生。对MFO系统的酶，如氨基比林脱甲基酶和芳烃羟化酶进行了测定，并测定了细胞色素P-450的含量。在接种Cy之前接种P（60微克/千克）比在接种Cy之后给予P效果更好。P作用的确切机制尚不清楚。经P处理的动物似乎比Cy组动物有更早修复由Cy的有毒代谢物造成损伤的能力。蛋白A的这一特性可能有助于在Cy代谢物产生毒性损伤后加速肝脏MFO系统中酶活性的再生。
