Raisuddin S, Parmar D, Zaidi S I, Singh K P, Verma A S, Seth P K, Ray P K
Immunotechnology Section, Bose Institute, Calcutta, India.
Eur J Drug Metab Pharmacokinet. 1994 Apr-Jun;19(2):163-8. doi: 10.1007/BF03188837.
Aflatoxins are suspected human carcinogens and are also known to possess diverse toxicological activities. In the present communication an attempt has been made to evaluate the effects of aflatoxin B1 (AFB1) on hepatic microsomal drug metabolizing enzymes in growing rats. The weanling rats were exposed to 60, 300 or 600 micrograms AFB1/kg body weight, per os, on alternate days for 4 weeks, in 0.2 ml corn oil. A significant depression in the activities of aryl hydrocarbon hydroxylase (AHH), aniline hydroxylase (AH) and aminopyrene-N-demethylase (AND) was observed at 300 micrograms and 600 micrograms doses of AFB1. However, no significant change was recorded in glutathione-S-transferase (GST) activity and total sulphydryl (SH) content upon AFB1 exposure in weanling rats. Thus, AFB1 appears to have more pronounced effect on the phase I, rather than phase II, biotransformation enzyme system in weanling rats. The depression of drug metabolizing enzymes together with suppression of immunity by AFB1, as reported earlier by us, may increase the susceptibility of the host to toxic chemicals, drugs and infectious agents, particularly during the post-natal period.
黄曲霉毒素被怀疑是人类致癌物，并且已知具有多种毒理学活性。在本报告中，已尝试评估黄曲霉毒素B1（AFB1）对生长中大鼠肝脏微粒体药物代谢酶的影响。将断奶大鼠以0.2 ml玉米油为溶剂，每隔一天经口给予60、300或600微克AFB1/千克体重，持续4周。在300微克和600微克剂量的AFB1处理下，观察到芳烃羟化酶（AHH）、苯胺羟化酶（AH）和氨基芘-N-脱甲基酶（AND）的活性显著降低。然而，断奶大鼠暴露于AFB1后，谷胱甘肽-S-转移酶（GST）活性和总巯基（SH）含量没有明显变化。因此，AFB1似乎对断奶大鼠的I相生物转化酶系统比对II相生物转化酶系统有更显著的影响。正如我们之前报道的，药物代谢酶的降低以及AFB1对免疫的抑制作用，可能会增加宿主对有毒化学物质、药物和传染原的易感性，尤其是在出生后阶段。
