Penton Z
Clin Chem. 1985 Mar;31(3):439-41.
An autosampler intended for liquid injection into a gas chromatograph was modified to serve as an automatic headspace sampler by replacing the syringe with a 100-microL gas-tight syringe and shortening the needle that normally enters the vials for sampling. Instead of the usual 1.0 to 1.5 mL of liquid sample, the vials contained 200 microL of liquid; the vapor above the liquid was injected into the gas chromatograph. Blood samples from California drivers were analyzed for ethanol by using the modified autosampler and the values were compared with those obtained by two other methods: the Smith modified version of the Widmark diffusion desiccation oxidation method (J Lab Clin Med 38: 762, 1951) and direct injection of diluted blood into the gas chromatograph. The correlation coefficient between the headspace method described in this paper and the titration method was 0.984; with direct injection, 0.997.
一种用于向气相色谱仪中注入液体的自动进样器进行了改装，通过将注射器换成100微升气密注射器并缩短通常用于进入样品瓶进行采样的针头，使其用作自动顶空进样器。样品瓶中装有200微升液体，而非通常的1.0至1.5毫升液体样品；液体上方的蒸汽被注入气相色谱仪。使用改装后的自动进样器对加利福尼亚州司机的血液样本进行乙醇分析，并将结果与通过其他两种方法获得的值进行比较：史密斯改良版的维德马克扩散干燥氧化法（《实验室与临床医学杂志》38: 762, 1951）以及将稀释后的血液直接注入气相色谱仪。本文所述顶空法与滴定法之间的相关系数为0.984；与直接进样法的相关系数为0.997。
