Schmidt Robert W, de Bakker Erik, Ariese Freek
LaserLaB, Vrije Universiteit Amsterdam, Amsterdam, Netherlands.
Department of Molecular Cell Biology and Immunology, and Plastic, Reconstructive and Hand Surgery, Amsterdam UMC, Location VUMC, Amsterdam, Netherlands.
J Biophotonics. 2025 Feb;18(2):e202400415. doi: 10.1002/jbio.202400415. Epub 2024 Dec 23.
Breast augmentations, commonly performed for aesthetic or medical reasons, often use silicone (polydimethylsiloxane [PDMS]) implants. Some patients develop complications like capsular contracture, where scar tissue forms around the implant. Previously, we used stimulated Raman scattering (SRS) microscopy to detect and quantify silicone in stained capsule tissue, finding a correlation between silicone amount and contracture severity. However, we suspected silicone loss during histological preparation, which includes multiple steps like formalin fixation and paraffin embedding. In this study, we assessed silicone loss by comparing adjacent tissue samples from the same capsule: one prepared conventionally and the other snap-frozen. SRS microscopy revealed that snap-frozen samples had roughly five times more silicone, indicating significant silicone loss during conventional preparation. Thus, measuring silicone in histologically prepared samples likely underestimates PDMS content.
隆胸手术通常出于美学或医学原因进行，常使用硅胶（聚二甲基硅氧烷[PDMS]）植入物。一些患者会出现如包膜挛缩等并发症，即植入物周围形成瘢痕组织。此前，我们使用受激拉曼散射（SRS）显微镜检测并量化染色包膜组织中的硅胶，发现硅胶含量与挛缩严重程度之间存在相关性。然而，我们怀疑在包括福尔马林固定和石蜡包埋等多个步骤的组织学制备过程中硅胶会流失。在本研究中，我们通过比较来自同一包膜的相邻组织样本评估硅胶流失情况：一个采用传统方法制备，另一个速冻。SRS显微镜显示，速冻样本中的硅胶含量大约多五倍，表明传统制备过程中硅胶有显著流失。因此，在组织学制备样本中测量硅胶含量可能会低估PDMS的含量。
