LaserLaB Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Department of Plastic, Reconstructive and Hand Surgery, Amsterdam UMC Location VUMC, Amsterdam Movement Sciences, Amsterdam, The Netherlands.
J Biophotonics. 2020 May;13(5):e201960197. doi: 10.1002/jbio.201960197. Epub 2020 Feb 23.
Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright-field images revealed the distribution and quantity of silicone material in the tissue. Fast and high-resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant-linked diseases.
全球数以百万计的女性都拥有硅胶乳房植入物。据报道，大约十分之一的患者在 10 年内会出现植入物失效，而硅胶碎片传播的后果还不太清楚。目前，组织病理学切片中的硅胶检测是基于形态学特征，因为没有可用的特定免疫组织化学技术。在这里，我们展示了受激拉曼散射（SRS）成像在不进行额外样品处理的情况下，特异性检测染色组织病理学切片中硅胶材料的可行性和灵敏度。从不同类型的植入物中取出假体周围囊后，获得了四个组织学切片，以及一名破裂植入物患者的腋窝淋巴结肿大。与明场图像配准的 SRS 图像显示了组织中硅胶材料的分布和数量。使用 SRS 对组织学切片进行快速和高分辨率的分子特异性成像，为研究硅胶碎片在与植入物相关疾病的病理生理学中的作用提供了机会。
