Bu Lina, Huang Shaodong, Rao Ziyan, Wu Chenyang, Sun Bryan-Yu, Liu Yanhua, He Lin, Zhao Dongyu
Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China.
Mol Ther Nucleic Acids. 2024 Nov 16;35(4):102397. doi: 10.1016/j.omtn.2024.102397. eCollection 2024 Dec 10.
CHD6, a member of the chromodomain helicase DNA-binding protein family, has been implicated in various diseases and tumors. However, its precise binding model of CHD6 on regulatory functional genes remains poorly understood. In this study, we discovered sharp peaks of CHD6, as the first member of CHD family for housekeeping process, binding only to the promoter region of genes in the C4-2 cell line. These genes, with conserved sharp CHD6 peaks across tumor cells, likely represent housekeeping genes and . Genes with sharp CHD6 peaks exhibit stable and low expression levels, sharing epigenetic features similar to housekeeping genes. Furthermore, this regulatory model also exists in both HEK293 cells and cardiomyocytes. Overall, the results of this study demonstrate that CHD6 binds to the promoter regions of housekeeping genes, regulating their histone modifications, chromatin structure, and gene expression.
CHD6是染色质结构域解旋酶DNA结合蛋白家族的成员，与多种疾病和肿瘤有关。然而，CHD6在调控功能基因上的确切结合模式仍知之甚少。在本研究中，我们发现作为看家过程中CHD家族的首个成员，CHD6的尖锐峰仅与C4-2细胞系中基因的启动子区域结合。这些基因在肿瘤细胞中具有保守的尖锐CHD6峰，可能代表看家基因。具有尖锐CHD6峰的基因表现出稳定且低的表达水平，具有与看家基因相似的表观遗传特征。此外，这种调控模式在HEK293细胞和心肌细胞中也存在。总体而言，本研究结果表明CHD6与看家基因的启动子区域结合，调节其组蛋白修饰、染色质结构和基因表达。
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