M Dakshinya, P Anitha, Smiline Girija A S, A Paramasivam, Priyadharsini J Vijayashree
Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, India.
Department of Microbiology, Centre for Infectious Diseases, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, India.
J Oral Biol Craniofac Res. 2025 Jan-Feb;15(1):48-56. doi: 10.1016/j.jobcr.2024.11.007. Epub 2024 Dec 5.
Periodontitis is considered to be one of the major risk factors associated with cancers of the oral cavity. Periodontogenic pathogens such as and are the important pathogens associated with periodontitis. Chronic exposure to bacterial components induces changes in the nearby cells. Hence, the present study has been designed to identify the molecular mechanisms that could be associated with the two disease conditions periodontitis and head and neck cancer.
The present study investigated the differential gene expression profile in human gingival keratinocytes treated with a bacterium associated with periodontal disease, and its possible association with the development of Head and Neck Squamous Cell Carcinoma (HNSCC).
The study followed a computational design. Multiple tools and databases, such as GEOmnibus, STRING, Metascape, PANTHER, and UALCAN, cBioportal, were used to derive an association between gene expression during infection with and the resulting gene expression profiles were analyzed in the HNSCC dataset.
The study revealed 29 genes from a pool of transcripts acquired after comparing the Pg-HIGK and Sham-HIGK. Among them, 3 genes i.e., and were found to be overexpressed and significantly influenced patient survival. The upregulation of was found to correlate with poor prognosis in HNSCC patients.
The study provided insights into the possible association of and in the development of HNSCC. Further investigations are warranted to confirm the functional role of these genes in establishing the cancer phenotype in patients with chronic infection with .
牙周炎被认为是与口腔癌相关的主要危险因素之一。牙龈卟啉单胞菌等牙周致病菌是与牙周炎相关的重要病原体。长期暴露于细菌成分会诱导附近细胞发生变化。因此，本研究旨在确定可能与牙周炎和头颈癌这两种疾病相关的分子机制。
本研究调查了用与牙周疾病相关的细菌牙龈卟啉单胞菌处理的人牙龈角质形成细胞中的差异基因表达谱，及其与头颈部鳞状细胞癌（HNSCC）发生发展的可能关联。
该研究采用了计算设计。使用了多种工具和数据库，如GEOmnibus、STRING、Metascape、PANTHER和UALCAN、cBioportal，来推导牙龈卟啉单胞菌感染期间的基因表达与HNSCC数据集中所得基因表达谱之间的关联。
该研究从比较牙龈卟啉单胞菌处理的人牙龈角质形成细胞（Pg-HIGK）和假处理的人牙龈角质形成细胞（Sham-HIGK）后获得的转录本库中发现了29个基因。其中，3个基因，即[具体基因名称1]、[具体基因名称2]和[具体基因名称3]被发现过度表达，并对患者生存有显著影响。发现[具体基因名称1]的上调与HNSCC患者的不良预后相关。
该研究为牙龈卟啉单胞菌与HNSCC发生发展的可能关联提供了见解。有必要进一步研究以证实这些基因在慢性牙龈卟啉单胞菌感染患者建立癌症表型中的功能作用。
