Loury D J, Byard J L
Environ Mutagen. 1985;7(2):245-54. doi: 10.1002/em.2860070211.
To investigate possible interspecies differences in the hepatocellular genotoxicity of the food-borne mutagens 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethyl-imidazo[4,5-f]quinoline (MeIQ), primary cultures of rat, hamster, and guinea pig hepatocytes were established. The induction of DNA repair activity in cultures exposed to various concentrations of IQ and MeIQ was determined by liquid scintillation spectrometry. DNA repair responses to MeIQ were, in general, greater than those elicited by IQ. In all three preparations of rat and hamster hepatocytes and in two of three preparations of guinea pig cells, MeIQ produced statistically significant (p less than 0.05) repair responses. IQ stimulated significant levels of repair in all three rat hepatocyte preparations and in two of three hamster cell preparations. In guinea pig cells exposed to IQ, no significant repair activity was observed. These results indicate that the genotoxicity of IQ and MeIQ in hepatic cells in species-dependent.
为了研究食源性诱变剂2-氨基-3-甲基咪唑[4,5-f]喹啉（IQ）和2-氨基-3,4-二甲基咪唑[4,5-f]喹啉（MeIQ）在肝细胞遗传毒性方面可能存在的种间差异，建立了大鼠、仓鼠和豚鼠肝细胞的原代培养物。通过液体闪烁光谱法测定暴露于不同浓度IQ和MeIQ的培养物中DNA修复活性的诱导情况。一般来说，对MeIQ的DNA修复反应大于IQ引起的反应。在大鼠和仓鼠肝细胞的所有三种制剂以及豚鼠细胞的三种制剂中的两种中，MeIQ产生了具有统计学意义（p小于0.05）的修复反应。IQ在所有三种大鼠肝细胞制剂和三种仓鼠细胞制剂中的两种中刺激了显著水平的修复。在暴露于IQ的豚鼠细胞中，未观察到显著的修复活性。这些结果表明，IQ和MeIQ在肝细胞中的遗传毒性具有种属依赖性。
