Malthiéry Y, Lissitzky S
Eur J Biochem. 1985 Feb 15;147(1):53-8. doi: 10.1111/j.1432-1033.1985.tb08717.x.
Thyroglobulin, the dimeric glycoprotein (19 S, 2 X 330 kDa), specific to the thyroid gland, is the support for thyroid hormone synthesis. Elucidation of the mechanism for thyroid hormone synthesis requires the knowledge of the primary sequence of the protein. In this paper the sequence of the first coding 2190 nucleotides from the 5' end of the human mRNA is presented. This was obtained by sequencing two previously described overlapping clones and by construction and sequencing of a single-stranded cDNA corresponding to the 5' end of the mRNA. The nucleotide sequence represents a quarter of the human thyroglobulin mRNA, from which a polypeptide sequence of 730 amino acids at the NH2-terminal end of the monomer has been deduced. This sequence shows a repetition of five highly conserved motifs each of approximately 50 amino acids, the analysis of which allowed us to establish a consensus sequence. We have also demonstrated (a) the hormonogenic tyrosine residue recently described in the mature protein, which is located four amino acids after the NH2-terminal Asn; (b) a prepeptide signal of thyroglobulin secretion comprising 19 amino acids preceding the Asn residue, the NH2-terminal residue of the mature protein and (c) a six-signal tripeptide (Asn-Xaa-Thr or Ser) of N-glycosylation of the chain.
甲状腺球蛋白是一种二聚体糖蛋白（19S，2×330 kDa），是甲状腺特有的，是甲状腺激素合成的支架。阐明甲状腺激素合成机制需要了解该蛋白质的一级序列。本文给出了人类mRNA 5'端前2190个编码核苷酸的序列。这是通过对两个先前描述的重叠克隆进行测序以及构建并测序对应于mRNA 5'端的单链cDNA获得的。核苷酸序列代表了人类甲状腺球蛋白mRNA的四分之一，由此推导出单体NH2末端730个氨基酸的多肽序列。该序列显示出五个高度保守基序的重复，每个基序约含50个氨基酸，对其分析使我们能够确定一个共有序列。我们还证实了：（a）最近在成熟蛋白中描述的激素生成酪氨酸残基，位于NH2末端天冬酰胺之后的四个氨基酸处；（b）甲状腺球蛋白分泌的前肽信号，由成熟蛋白NH2末端残基天冬酰胺之前的19个氨基酸组成；以及（c）该链N-糖基化的六个信号三肽（天冬酰胺-Xaa-苏氨酸或丝氨酸）。
