Li Ang, Li Jiaqi, Wang Yingpu, Liu Zirong, Liu Lulei, Liu Longzhen, Xue Suyan, Zhu Ling, Mao Yuze
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, 266237, China.
Heliyon. 2024 Dec 5;10(23):e40921. doi: 10.1016/j.heliyon.2024.e40921. eCollection 2024 Dec 15.
The low temperatures in winter, particularly the cold spells in recent years, have posed significant threats to China's abalone aquaculture industry. The low temperature tolerance of cultured abalone has drawn plenty of attention, but the metabolic response of abalone to low-temperature stress remains unclear. In this study, we investigated the metabolomic analysis of Pacific abalone () during low-temperature stress. Pacific abalone used two strains of cultured abalone, namely the bottom-sowing cultured strain (DB) and the longline cultured strain (FS), which had different histories of low-temperature acclimation. The results revealed that eight of the top 10 shared differential expression metabolites of the two strains were carbohydrates. According to the results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis, low-temperature stress primarily affected several metabolic pathways. These pathways include ABC transporters, carbohydrate digestion and absorption, starch and sucrose metabolism, lysine degradation, TCA cycle, the phosphotransferase system, the glucagon signaling pathway and pyruvate metabolism. The results suggest that Pacific abalone primarily regulates the expression of carbohydrates to enhance energy supply and anti-freezing protection. These findings are crucial for understanding the mechanism of low-temperature tolerance in Pacific abalone, and can help optimize culture strategies for high-quality abalone aquaculture development.
冬季的低温，尤其是近年来的寒潮，对中国的鲍鱼养殖业构成了重大威胁。养殖鲍鱼的低温耐受性已引起广泛关注，但鲍鱼对低温胁迫的代谢反应仍不清楚。在本研究中，我们调查了低温胁迫期间太平洋鲍鱼（）的代谢组学分析。太平洋鲍鱼使用了两种养殖鲍鱼品系，即底播养殖品系（DB）和延绳养殖品系（FS），它们具有不同的低温驯化历史。结果显示，两个品系前10个共享差异表达代谢物中有8个是碳水化合物。根据京都基因与基因组百科全书（KEGG）代谢途径分析结果，低温胁迫主要影响了几个代谢途径。这些途径包括ABC转运蛋白、碳水化合物消化和吸收、淀粉和蔗糖代谢、赖氨酸降解、三羧酸循环、磷酸转移酶系统、胰高血糖素信号通路和丙酮酸代谢。结果表明，太平洋鲍鱼主要通过调节碳水化合物的表达来增强能量供应和抗冻保护。这些发现对于理解太平洋鲍鱼的低温耐受机制至关重要，并有助于优化养殖策略，以促进优质鲍鱼养殖的发展。
