在杂交水稻中构建具有正常种子生产能力的高频无融合生殖体系。

Engineering high-frequency apomixis with normal seed production in hybrid rice.

作者信息

Song Minglei, Li Fei, Chen Zhen, Hou Haonan, Wang Yu, Liu Huixia, Liu Di, Li Junzhou, Peng Ting, Zhao Yafan, Du Yanxiu, Sun Hongzheng, Du Changqing, Zhang Jing, Zhao Quanzhi, Miao Chunbo

机构信息

Henan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.

Institute of Advanced Biotechnology and School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.

出版信息

iScience. 2024 Nov 26;27(12):111479. doi: 10.1016/j.isci.2024.111479. eCollection 2024 Dec 20.

DOI:10.1016/j.isci.2024.111479

PMID:39720515

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11667186/

Abstract

Clonal reproduction through seeds, also termed apomixis, has the potential to revolutionize agriculture by allowing hybrid crops to be clonally propagated. Although apomixis has been introduced into rice through engineering in recent years, the poor fertility and low-frequency clonal reproduction of synthetic apomicts hinder the application of apomixis in crop breeding. Here, in elite hybrid rice, we generated many apomicts, which produced clonal progeny with frequencies of > 95.0% and had high even normal fertility through combining enhanced rice BABYBOOM1 (BBM1)-induced parthenogenesis with the simultaneous inactivation of , , and . These synthetic apomicts maintained the agronomic traits of the elite hybrid rice. Our results indicate that fertility-normal hybrids with high penetrance of apomixis can be generated in rice, thereby laying an important foundation for the application of synthetic apomixis in crop breeding.

摘要

通过种子进行的克隆繁殖，也称为无融合生殖，有可能通过使杂交作物能够进行克隆繁殖来彻底改变农业。尽管近年来已通过基因工程将无融合生殖引入水稻，但合成无融合生殖体的育性差和克隆繁殖频率低阻碍了无融合生殖在作物育种中的应用。在此，我们在优良杂交水稻中培育出了许多无融合生殖体，通过将增强型水稻BABYBOOM1（BBM1）诱导的孤雌生殖与、和的同时失活相结合，这些无融合生殖体产生克隆后代的频率>95.0%，并且具有高甚至正常的育性。这些合成无融合生殖体保持了优良杂交水稻的农艺性状。我们的结果表明，在水稻中可以产生具有高无融合生殖渗透率的育性正常的杂种，从而为合成无融合生殖在作物育种中的应用奠定了重要基础。