Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, 201602, Shanghai, China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, 311300, Lin'an, Hangzhou, China.
Nat Commun. 2019 Aug 23;10(1):3822. doi: 10.1038/s41467-019-11830-5.
The widespread agricultural problem of pre-harvest sprouting (PHS) could potentially be overcome by improving seed dormancy. Here, we report that miR156, an important grain yield regulator, also controls seed dormancy in rice. We found that mutations in one MIR156 subfamily enhance seed dormancy and suppress PHS with negligible effects on shoot architecture and grain size, whereas mutations in another MIR156 subfamily modify shoot architecture and increase grain size but have minimal effects on seed dormancy. Mechanistically, mir156 mutations enhance seed dormancy by suppressing the gibberellin (GA) pathway through de-represssion of the miR156 target gene Ideal Plant Architecture 1 (IPA1), which directly regulates multiple genes in the GA pathway. These results provide an effective method to suppress PHS without compromising productivity, and will facilitate breeding elite crop varieties with ideal plant architectures.
广泛存在于农业领域的采前发芽（PHS）问题，可能通过改善种子休眠来解决。在这里，我们报告称，miR156 作为一种重要的谷物产量调节剂，同样控制着水稻种子的休眠。我们发现，一个 MIR156 亚家族的突变增强了种子休眠并抑制了 PHS，对芽结构和谷物大小几乎没有影响，而另一个 MIR156 亚家族的突变改变了芽结构并增加了谷物大小，但对种子休眠的影响最小。从机制上讲，mir156 突变通过去抑制 miR156 靶基因理想植物结构 1（IPA1）来增强种子休眠，IPA1 直接调控 GA 途径中的多个基因。这些结果提供了一种在不影响生产力的情况下抑制 PHS 的有效方法，并将促进具有理想植物结构的优良作物品种的培育。
