Abe Harue, Ueno Saneyoshi, Matsuo Ayumi, Hirota Shun K, Miura Hiroki, Su Mong-Huai, Shen Yun-Guang, Tsumura Yoshihiko, Suyama Yoshihisa, Wang Zhong-Lang
Center for Sustainable Agriculture and Forestry, Faculty of Agriculture Niigata University Sado Niigata Japan.
Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute Forest Research and Management Organization Tsukuba Ibaraki Japan.
Ecol Evol. 2024 Dec 24;14(12):e70721. doi: 10.1002/ece3.70721. eCollection 2024 Dec.
The genus is widely distributed, primarily in East Asia. is located at the northern limit of this genus distribution, and understanding changes in its distribution is crucial for understanding the evolution of plants in this region, as well as their relationship with geological history and climate change. Moreover, the classification of sect. in Japan has not been clarified. Therefore, this study aims to understand the evolutionary history of the Japanese sect. . The genetic population structure was analysed using SNP data and MIG-seq. The relationship between the Japanese sect. , including the related species in China, was further inferred from the phylogeny generated by RA x ML, SplitsTree and PCA. Population genetic structure was inferred using a Bayesian clustering method (ADMIXTURE). We subsequently employed approximate Bayesian computation, which was further supported by the coalescent simulations (DIYABC, fastsimcoal and Bayesian Skyline Plots) to explore the changes in population, determining which events appropriately explain the phylogeographical signature. Ecological niche modelling was combined with genetic analyses to compare current and past distributions. The analyses consistently showed that and are distinct, having diverged from each other during the Middle to Late Miocene period. Furthermore, differentiated into four major populations (North, South, Ryukyu-Taiwan and Continent). The Japanese sect. underwent speciation during archipelago formation, reflecting its ancient evolutionary history compared with other native Japanese plants. did not diverge from in snow-rich environments during the Quaternary period. Our results suggest that both species have been independent since ancient times and that ancestral populations of have persisted in northern regions. Furthermore, the population on the continent is hypothesised to have experienced a reverse-colonisation event from southern Japan during the late Pleistocene glaciation.
该属分布广泛，主要分布在东亚。[具体物种名称]位于该属分布的北界，了解其分布变化对于理解该地区植物的进化以及它们与地质历史和气候变化的关系至关重要。此外，日本[具体物种名称]组的分类尚未明确。因此，本研究旨在了解日本[具体物种名称]组的进化历史。使用SNP数据和MIG-seq分析遗传种群结构。从RAxML、SplitsTree和PCA生成的系统发育中进一步推断日本[具体物种名称]组与中国相关物种之间的关系。使用贝叶斯聚类方法（ADMIXTURE）推断种群遗传结构。随后，我们采用近似贝叶斯计算，并通过溯祖模拟（DIYABC、fastsimcoal和贝叶斯天际线图）进一步支持，以探索种群变化，确定哪些事件能够恰当地解释系统地理学特征。将生态位建模与遗传分析相结合，以比较当前和过去的分布。分析一致表明，[具体物种名称1]和[具体物种名称2]是不同的，它们在中新世中期至晚期彼此分化。此外，[具体物种名称1]分化为四个主要种群（北部、南部、琉球-台湾和大陆）。日本[具体物种名称]组在群岛形成过程中经历了物种形成，与其他日本本土植物相比，反映了其古老的进化历史。[具体物种名称1]在第四纪时期没有在积雪丰富的环境中与[具体物种名称2]分化。我们的结果表明，这两个物种自古以来就是独立的，并且[具体物种名称1]的祖先种群一直存在于北部地区。此外，假设大陆上的[具体物种名称1]种群在晚更新世冰川期经历了从日本南部的反向殖民事件。
