Nemeth P R, Daly K, Erde S, Wood J D
Experientia. 1985 Feb 15;41(2):259-61. doi: 10.1007/BF02002626.
Intracellular recording methods were used to investigate the action of methylene blue on electrical behavior of myenteric neurons in guinea pig small intestine. The neurophysiological studies were done in parallel with studies on contractile activity of the intestinal musculature. Methylene blue depolarized the membranes, increased the input resistance, augmented excitability and reduced postspike hyperpolarizing potentials in AH/Type 2 myenteric neurons. These effects, with the exception of suppression of postspike hyperpolarization, were reversed by exposure to elevated calcium. The mechanism of action of methylene blue appeared to be suppression of calcium-dependent potassium conductance in the neuronal membranes. The neuronal action of methylene blue was manifest as a release of excitatory neurontransmitter substances which evoked contraction of the small intestinal longitudinal muscle.
采用细胞内记录方法研究亚甲蓝对豚鼠小肠肌间神经元电活动的作用。神经生理学研究与肠道肌肉收缩活动的研究同时进行。亚甲蓝使AH/2型肌间神经元的膜去极化，增加输入电阻，增强兴奋性并降低锋电位后超极化电位。除了抑制锋电位后超极化外，这些效应在暴露于高钙时会逆转。亚甲蓝的作用机制似乎是抑制神经元膜中钙依赖性钾电导。亚甲蓝对神经元的作用表现为兴奋性神经递质物质的释放，从而引起小肠纵肌收缩。
