Raymond V, Leung P C, Veilleux R, Labrie F
FEBS Lett. 1985 Mar 11;182(1):196-200. doi: 10.1016/0014-5793(85)81183-2.
In cultured rat anterior pituitary cells, the agonist [Asu1,6, Arg8]vasopressin (AVP-A) increased by 1.5-fold 32Pi incorporation into phosphatidic acid (PA), as early as 15 s after its addition. Increased phosphatidylinositol (PI) labeling became significant 4 min after AVP-A addition. Dose-response measurements with AVP-A showed ED50 values of 76 and 62 nM for PA and PI labeling, respectively. Peptide corticotropin-releasing factor (CRF) (0.1 microM) did not affect the stimulatory effect of AVP-A on PA and PI labeling. These data suggest that stimulation of PI metabolism in corticotrophs may be one of the early events involved in the stimulation of ACTH release induced by vasopressin.
在培养的大鼠垂体前叶细胞中，激动剂[Asu1,6, Arg8]加压素（AVP - A）早在添加后15秒就使32Pi掺入磷脂酸（PA）的量增加了1.5倍。添加AVP - A 4分钟后，磷脂酰肌醇（PI）标记增加变得显著。用AVP - A进行的剂量反应测量显示，PA和PI标记的半数有效剂量（ED50）值分别为76和62 nM。肽促肾上腺皮质激素释放因子（CRF）（0.1 microM）不影响AVP - A对PA和PI标记的刺激作用。这些数据表明，刺激促肾上腺皮质激素细胞中的PI代谢可能是血管加压素诱导促肾上腺皮质激素释放刺激所涉及的早期事件之一。
