精氨酸加压素刺激大鼠睾丸间质细胞中磷脂酸的形成。

Arginine-vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells.

作者信息

Nielsen J R, Hansen H S, Jensen B

出版信息

FEBS Lett. 1987 Jun 22;218(1):93-6. doi: 10.1016/0014-5793(87)81025-6.

Abstract

Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [14C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10(-6)M AVP [14C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10(-11)-10(-6)M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V1 antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V2 agonist dPVDAVP was without effect. The present results suggest that AVP binds to V1 receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.

摘要

精氨酸加压素（AVP）刺激了用[14C]花生四烯酸预标记的大鼠睾丸间质细胞中标记磷脂酸（PA）的形成。加入10(-6)M AVP后，[14C]花生四烯酰磷脂酸在2分钟内达到最大值。这种增加呈剂量依赖性（10(-11)-10(-6)M）。未检测到其他磷脂和二酰基甘油的标记变化。V1拮抗剂dPTyr(Me)AVP以剂量依赖性方式抑制AVP刺激的PA积累。V2激动剂dPVDAVP无作用。目前的结果表明，AVP与大鼠睾丸间质细胞中的V1受体结合，导致PA周转的刺激。我们认为，AVP刺激的PA形成是磷脂酰肌醇周转的一个指标。