Nagao Munemasa, Fukuda Akihisa, Kashima Hirotaka, Matsuyama Sho, Iimori Kei, Nakayama Shinnosuke, Mizukoshi Kenta, Kawai Munenori, Yamakawa Go, Omatsu Mayuki, Namikawa Mio, Masuda Tomonori, Hiramatsu Yukiko, Muta Yu, Maruno Takahisa, Nakanishi Yuki, Tsuruyama Tatsuaki, Seno Hiroshi
Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan; Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Eur J Cell Biol. 2025 Mar;104(1):151472. doi: 10.1016/j.ejcb.2024.151472. Epub 2024 Dec 19.
The biliary tract is a ductal network comprising the intrahepatic (IHBDs) and extrahepatic bile duct (EHBDs). Biliary duct disorders include cholangitis, neoplasms, and injury. However, the underlying mechanisms are not fully understood. With advancements in 3D culture technology, cholangiocyte organoids (COs) derived from primary tissues or induced pluripotent stem cells (iPSCs) can accurately replicate the structural and functional properties of biliary tissues. These organoids have become powerful tools for studying the pathogenesis of biliary diseases, such as cystic fibrosis and primary sclerosing cholangitis, and for developing new therapeutic strategies for cholangiocarcinoma. Additionally, COs have the potential to repair bile duct injuries and facilitate transplantation therapies. This review also discusses the use of organoids in genetically engineered mouse models to provide mechanistic insights into tumorigenesis and cancer progression. Continued innovation and standardization of organoid technology are crucial for advancing precision medicine for biliary diseases and cancer.
胆道是一个由肝内胆管（IHBDs）和肝外胆管（EHBDs）组成的导管网络。胆管疾病包括胆管炎、肿瘤和损伤。然而，其潜在机制尚未完全明确。随着3D培养技术的进步，源自原代组织或诱导多能干细胞（iPSCs）的胆管细胞类器官（COs）能够准确复制胆管组织的结构和功能特性。这些类器官已成为研究胆管疾病（如囊性纤维化和原发性硬化性胆管炎）发病机制以及开发胆管癌新治疗策略的有力工具。此外，COs具有修复胆管损伤并促进移植治疗的潜力。本综述还讨论了类器官在基因工程小鼠模型中的应用，以提供对肿瘤发生和癌症进展的机制性见解。类器官技术的持续创新和标准化对于推进胆管疾病和癌症的精准医学至关重要。
