正常和延迟着床小鼠胚胎中的肌动蛋白信使核糖核酸含量

Actin mRNA content in normal and delayed implanting mouse embryos.

作者信息

Giebelhaus D H, Weitlauf H M, Schultz G A

出版信息

Dev Biol. 1985 Feb;107(2):407-13. doi: 10.1016/0012-1606(85)90322-7.

Abstract

Actin mRNA levels were measured in mouse eggs, early embryos, and delayed implanting blastocysts by a homologous, cloned recombinant DNA probe and "dot" blot methodology. A maternal store of 431 fg of actin mRNA was observed in the unfertilized eggs. This mRNA pool decreased 12-fold by the mid-two-cell stage. Actin mRNA levels were then observed to increase progressively from the eight-cell to the blastocyst stage on a basis proportional to cell number. Late blastocysts contained 2400 fg actin mRNA per embryo (22 fg per cell). The cellular level decreased by about 20% in embryos induced into delay of implantation by ovariectomy of donor females. Reactivation of the delayed implanting blastocysts through hormonal manipulation in vivo or culture in vitro was accompanied by reestablishment of the level of cellular actin mRNA observed in normal blastocysts.

摘要

采用同源克隆重组DNA探针和“斑点”印迹法，测定了小鼠卵子、早期胚胎以及延迟着床囊胚中的肌动蛋白mRNA水平。在未受精卵中观察到431fg的肌动蛋白mRNA母源储备。到二细胞中期时，该mRNA库减少了12倍。随后观察到，从八细胞期到囊胚期，肌动蛋白mRNA水平随细胞数量按比例逐渐增加。晚期囊胚每个胚胎含有2400fg肌动蛋白mRNA（每个细胞22fg）。供体雌性小鼠卵巢切除诱导胚胎延迟着床，胚胎中的细胞水平下降约20%。通过体内激素处理或体外培养使延迟着床囊胚重新激活，伴随着恢复到正常囊胚中观察到的细胞肌动蛋白mRNA水平。