Gangemi Christina G, Janovjak Harald
Flinders Health and Medical Research Institute (FHMRI), College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australia.
Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC, Australia.
Methods Mol Biol. 2025;2840:231-244. doi: 10.1007/978-1-0716-4047-0_17.
Optogenetic experiments rely on the controlled delivery of light to diverse biological systems. Impressive devices have been recently developed to stimulate cells and small animals with multiple wavelengths and intensities. However, existing hardware solutions are often limited to a single sample holder, and their design and cost can further limit scalability. This chapter describes an illumination system that is modular (through the use of accessible components) and scalable (through a shelving structure and low cost). Assembly and operation require no or minimal electrical engineering or programming expertise. Multi-intensity wavelength and temporal experiments can be performed in dozens of small and large samples. This chapter also introduces methods for temperature and light intensity measurements toward appropriate illumination conditions. This work aims to provide a greater level of accessibility and complementary opportunities for large-scale optogenetics in a broad range of biological samples.
光遗传学实验依赖于将光可控地传递到各种生物系统。最近已经开发出了令人印象深刻的设备，用于以多种波长和强度刺激细胞和小动物。然而，现有的硬件解决方案通常限于单个样品架，其设计和成本可能会进一步限制可扩展性。本章介绍了一种模块化（通过使用易于获取的组件）且可扩展（通过搁架结构和低成本）的照明系统。组装和操作不需要或仅需要极少的电气工程或编程专业知识。可以在数十个大小样本中进行多强度波长和时间实验。本章还介绍了用于测量温度和光强度以实现适当照明条件的方法。这项工作旨在为广泛的生物样本中的大规模光遗传学提供更高的可及性和补充机会。
