Vessière-Louveaux F, Darville M, Knuth A, Boon T
Int J Cancer. 1985 Feb 15;35(2):231-5. doi: 10.1002/ijc.2910350215.
By using irradiated BALB/c 3T3 mouse fibroblasts as feeder cells, we obtained a marked increase in the cloning efficiency of the 9 human melanoma cell lines that were tested. Four of these melanoma lines had a cloning efficiency that was lower than 0.0007 in the absence of feeder cells. In the presence of irradiated 3T3 cells, their cloning efficiency ranged from 0.017 to 0.26. For most melanoma cell lines the growth rate of cultures that were seeded at a low cell density was also increased by the use of 3T3 feeder cells. In high-density cultures, no effect was observed with most cell lines. The 3T3 effect was reversible. The pattern of surface antigens expressed by melanoma cells grown in the presence of 3T3 was similar to that observed without 3T3. The effect of feeder cells on the adaptation to culture of 5 primary melanomas was also tested. A definite improvement in cell yield was obtained with 4 of these melanomas.
通过使用经辐照的BALB/c 3T3小鼠成纤维细胞作为饲养细胞，我们测试的9种人类黑色素瘤细胞系的克隆效率显著提高。其中4种黑色素瘤细胞系在没有饲养细胞的情况下克隆效率低于0.0007。在有经辐照的3T3细胞存在时，它们的克隆效率在0.017至0.26之间。对于大多数黑色素瘤细胞系，使用3T3饲养细胞也提高了以低细胞密度接种的培养物的生长速率。在高密度培养中，大多数细胞系未观察到影响。3T3的作用是可逆的。在有3T3存在的情况下生长的黑色素瘤细胞表达的表面抗原模式与没有3T3时观察到的相似。还测试了饲养细胞对5种原发性黑色素瘤适应培养的影响。其中4种黑色素瘤的细胞产量有明显提高。
