Chanchaithong Pattrarat, Sroithongkham Parinya, Leelapsawas Chavin, Pinpimai Komkiew, Yindee Jitrapa, Collaud Alexandra, Perreten Vincent
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand; Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland; Research Unit in Microbial Food Safety and Antimicrobial Resistance, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand.
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand.
Vet Microbiol. 2025 Feb;301:110338. doi: 10.1016/j.vetmic.2024.110338. Epub 2024 Dec 5.
NDM-5-producing Escherichia coli are the predominant carbapenemase producers of medical and public health importance. The global spread of bla-containing plasmids in high-risk E. coli clones has been primarily documented in humans and increasingly reported in animals and the environment. Here, we used whole-genome sequence analysis to describe the genetic diversity of 16 high-risk NDM-5-producing E. coli strains, with a variety of NDM-5 plasmids, isolated from dogs and cats with extraintestinal infections in Thailand between 2017 and 2021. The strains belonged to sequence type (ST) 410 (n = 8), ST354 (n = 3), ST648 (n = 2), ST361 (n = 1), ST617 (n = 1), and ST641 (n = 1). The ST641 strain carried bla on an IncX3 plasmid, while bla was localized on single-replicon or multi-replicon IncF plasmids in other STs. Non-conjugative F1:A1:B49 NDM-5 plasmids were limited to ST410 strains. They contained bla associated with the IS26-bounded complex class 1 integron (Int1) with dfrA17-aadA5-qacEΔ1-sul1 cassette array that shared similarities to nearly identical structures with the plasmids of ST410 strains from humans in Thailand and Myanmar. Conjugative IncFII (F2:A-:B-) NDM-5 plasmids containing Int1 with dfrA12-aadA2-qacEΔ1-sul1 cassette array were present in ST354 and ST648, and heterogeneous plasmid STs of conjugative multi-replicon IncF NDM-5 plasmids were found in ST361, ST410, ST617, and ST648. The bla elements mobilized by IS26 were shared among various IncF plasmids in high-risk E. coli clones but were conserved within the endemic E. coli ST410, representing the predominant lineage in Southeast Asian countries. Dogs and cats can develop infections with NDM-5-producing E. coli, posing the risk of further disseminating carbapenemase in veterinary settings and the community. This emphasizes the need to implement infection control and antimicrobial resistance surveillance programs in veterinary settings.
产NDM-5的大肠杆菌是具有医学和公共卫生重要性的主要碳青霉烯酶产生菌。含bla的质粒在高危大肠杆菌克隆中的全球传播主要在人类中被记录，并且越来越多地在动物和环境中被报道。在此，我们使用全基因组序列分析来描述2017年至2021年期间从泰国患有肠道外感染的犬猫中分离出的16株携带多种NDM-5质粒的高危产NDM-5大肠杆菌菌株的遗传多样性。这些菌株属于序列型（ST）410（n = 8）、ST354（n = 3）、ST648（n = 2）、ST361（n = 1）、ST617（n = 1）和ST641（n = 1）。ST641菌株在一个IncX3质粒上携带bla，而bla在其他STs的单复制子或多复制子IncF质粒上定位。非接合性F1:A1:B49 NDM-5质粒仅限于ST410菌株。它们含有与IS26边界复合1类整合子（Int1）相关的bla以及dfrA17-aadA5-qacEΔ1-sul1盒式阵列，该阵列与来自泰国和缅甸人类的ST410菌株的质粒具有几乎相同结构的相似性。含有Int1以及dfrA12-aadA2-qacEΔ1-sul1盒式阵列的接合性IncFII（F2:A-:B-）NDM-5质粒存在于ST354和ST648中，并且在ST361、ST410、ST617和ST648中发现了接合性多复制子IncF NDM-5质粒的异质性质粒STs。由IS26移动的bla元件在高危大肠杆菌克隆的各种IncF质粒之间共享，但在地方性大肠杆菌ST410内保守，ST410是东南亚国家的主要谱系。犬猫可感染产NDM-5的大肠杆菌，这在兽医环境和社区中带来了碳青霉烯酶进一步传播的风险。这强调了在兽医环境中实施感染控制和抗菌药物耐药性监测计划的必要性。
