Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, South Korea.
Microb Drug Resist. 2020 Aug;26(8):1005-1008. doi: 10.1089/mdr.2019.0382. Epub 2020 Feb 11.
Carbapenemase-producing Enterobacteriaceae (CPE) have been very rarely reported in companion animals in South Korea. In this study, we aimed to investigate the molecular characteristics and relatedness of two New Delhi metallo-β-lactamase (NDM-5)-producing isolates from rectal swabs of a dog and a cat hospitalized in different veterinary hospitals in South Korea during 2019. Antimicrobial susceptibility was tested by the Etest and broth microdilution method. PCR and sequencing were performed to detect antimicrobial resistance genes. Plasmid replicon typing and Southern blotting hybridization were performed to determine the replication origin of the plasmid and location of the gene, respectively. Their macrorestriction profiles for isolates were assessed by multilocus sequence typing (MLST) and pulse-field gel electrophoresis (PFGE). The two carbapenem-resistant isolates harbored the gene located on the IncX3 plasmid. Allele sequence analysis for MLST showed that the two isolates were attributed to sequence type 410 (ST410). The NDM-5-producing isolate from the cat presented high clonal similarity (94%) assessed by PFGE to a previously reported NDM-5-producing ST410 isolate from a dog hospitalized in the same hospital in 2017. The two isolates for the genetic environment surrounding the gene had the same structure: IS-----IS. This study revealed a direct transmission of the NDM-5-producing ST410 isolate between a dog and a cat. This is the first report of NDM-5 carbapenemase-producing solate from a cat in South Korea.
在韩国，很少有关于宠物伴侣动物中产碳青霉烯酶肠杆菌科（CPE）的报道。在本研究中，我们旨在调查 2019 年在韩国两家不同兽医医院住院的犬和猫的直肠拭子中分离出的两株新型德里金属β-内酰胺酶（NDM-5）产生的分离株的分子特征和相关性。通过 Etest 和肉汤微量稀释法进行药敏试验。PCR 和测序用于检测抗生素耐药基因。质粒复制子分型和 Southern 印迹杂交分别用于确定质粒的复制原点和 基因的位置。通过多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）评估分离株的宏观限制图谱。两种碳青霉烯类耐药的分离株携带位于 IncX3 质粒上的 基因。MLST 的等位基因序列分析表明，两种 分离株均属于序列型 410（ST410）。来自猫的产 NDM-5 的 分离株与 2017 年在同一家医院住院的犬中产 NDM-5 的 ST410 分离株具有 94%的高克隆相似性（通过 PFGE 评估）。围绕 基因的两种 分离株的遗传环境具有相同的结构：IS-----IS。本研究揭示了 NDM-5 产 ST410 分离株在犬和猫之间的直接传播。这是在韩国首次报道产 NDM-5 碳青霉烯酶的猫分离株。
