McLaren J W, Brubaker R F
Invest Ophthalmol Vis Sci. 1985 Feb;26(2):144-52.
For certain studies of the dynamics of fluorescein in the anterior segment of the eye it would be advantageous to measure fluorescent intensity from several anatomic regions of the cornea and anterior chamber simultaneously or in rapid succession. In the present paper we describe a device called a two-dimensional scanning ocular fluorophotometer that we have designed and built to measure fluorescein concentration in a horizontal section through the cornea and anterior chamber. The 488-nm wavelength beam of an argon laser is mechanically scanned through the target area (70 microW total power at the eye) and fluorescent light is measured with a photon-counting photomultiplier tube. A single anterior-posterior scan requires 100 msec and is divided into 33 sample time periods of 3 msec each. Thirty anterior-posterior scans are made within 3 sec. A two-dimensional array of fluorescein concentration is reconstructed from the data. Concentrations ranging from 3 X 10(-10) gm/ml to 3 X 10(-6) g/ml are measured in a single scan. Examples of scans through the anterior chamber and cornea after topical and systemic administration of fluorescein are presented. These scans illustrate how this instrument can be used to measure two types of regional differences in fluorescein concentration, the "pupillary aqueous bubble" following topical administration and the radial concentration gradient in the cornea following systemic administration.
对于某些关于荧光素在前房动态变化的研究，若能同时或快速连续地测量角膜和前房几个解剖区域的荧光强度将大有益处。在本文中，我们描述了一种名为二维扫描眼荧光光度计的设备，它是我们设计并制造的，用于测量通过角膜和前房的水平截面中的荧光素浓度。氩离子激光的488纳米波长光束通过机械方式扫描目标区域（到达眼部时总功率为70微瓦），并用光子计数光电倍增管测量荧光。一次前后扫描需要100毫秒，分为33个3毫秒的采样时间段。在3秒内进行30次前后扫描。根据这些数据重建荧光素浓度的二维阵列。单次扫描可测量范围从3×10⁻¹⁰克/毫升至3×10⁻⁶克/毫升的浓度。文中给出了局部和全身应用荧光素后通过前房和角膜的扫描示例。这些扫描展示了该仪器如何用于测量荧光素浓度的两种区域差异，即局部应用后的“瞳孔水样泡”和全身应用后角膜中的径向浓度梯度。
