Gonçalves Fabiano Palmeira, Vieira-da-Motta Olney, Scelza Neto Pantaleo, Samarão Solange Silva, Tavares Sandro Junio de Oliveira, Seabra Sérgio Henrique, Taveira Gabriel Bonan, Scelza Miriam Fatima Zaccaro
Postgraduate Program in Dentistry of Fluminense Federal University (UFF), Niteroi, Rio de Janeiro, Brazil.
Animal Health Laboratory, Center for Agricultural Sciences and Technologies, University Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.
J Endod. 2025 Mar;51(3):303-315. doi: 10.1016/j.joen.2024.12.009. Epub 2024 Dec 24.
This study assessed a new antimicrobial paste formulation containing nitrofurantoin for regenerative endodontics against multispecies biofilms.
Four groups of 11 single-root teeth each were tested: Negative control group (brain-heart infusion broth + micro-organisms); Hoshino 1 conventional tri-antibiotic paste group (1:1:1 ratio); Hoshino 5 group (5:5:5 ratio); Experimental group with nitrofurantoin, ciprofloxacin, and metronidazole. Minimum inhibitory concentrations of each antimicrobial were determined. A polymicrobial biofilm (Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans) was created in root canals, and pastes were inserted for 3 minutes with lentulo. After 7 days, samples were sonicated and spectrophotometrically analyzed. Microbiological assays, plasma membrane permeabilization, confocal laser scanning microscopy, and scanning electron microscopy evaluated biofilm eradication. Statistical significance was set at P < .05.
Spectrophotometry indicated Experimental group outperformed Hoshino 1 group and Negative control group (P < .05), with results similar to Hoshino 5 group (P > .05). Experimental group showed increased membrane damage compared to Hoshino 1 group and Hoshino 5 group. Confocal laser scanning microscopy and scanning electron microscopy revealed fewer microorganisms and no residual biofilm in Experimental group.
Substituting minocycline with nitrofurantoin in the paste effectively reduced microorganism counts in root canals system.
本研究评估了一种含呋喃妥因的新型抗菌糊剂配方用于再生牙髓治疗对抗多种微生物生物膜的效果。
对四组每组11颗单根牙进行测试：阴性对照组（脑心浸液肉汤+微生物）；星野1传统三联抗生素糊剂组（1:1:1比例）；星野5组（5:5:5比例）；含呋喃妥因、环丙沙星和甲硝唑的实验组。测定每种抗菌剂的最低抑菌浓度。在根管中形成多微生物生物膜（粪肠球菌、金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌），并用慢锥钻将糊剂插入3分钟。7天后，对样本进行超声处理并进行分光光度分析。通过微生物学检测、质膜通透性、共聚焦激光扫描显微镜和扫描电子显微镜评估生物膜根除情况。统计学显著性设定为P<0.05。
分光光度法表明实验组优于星野1组和阴性对照组（P<0.05），结果与星野5组相似（P>0.05）。与星野1组和星野5组相比，实验组显示出膜损伤增加。共聚焦激光扫描显微镜和扫描电子显微镜显示实验组中的微生物较少且无残留生物膜。
在糊剂中用呋喃妥因替代米诺环素可有效减少根管系统中的微生物数量。
