Chandra Aakriti, Thosar Nilima
Department of Pediatric and Preventive Dentistry, Sharad Pawar Dental College and Hospital, Datta Meghe Institute of Higher Education and Research (Deemed to be University), Sawangi Meghe, Wardha, Maharastra, 442001, India.
F1000Res. 2024 Jan 11;12:754. doi: 10.12688/f1000research.133458.2. eCollection 2023.
Root canal infection in primary teeth is polymicrobial in nature. Most resistant micro-organisms, such as survive in chronic infection of the root canal at the periapical area and are difficult to remove by various root canal irrigants. has been found in chronic infections of root canals of primary teeth due to its ability to invade dentinal tubules. The multiple bacteria strains which cause endodontic infections, make it difficult to clean root canals with a single effective antibiotic. Hence Triple Antibiotic Paste (TAP) has been previously introduced, consisting of ciprofloxacin, metronidazole, and minocycline. Although this mixture aids in pulp regeneration and has been used to disinfect root canal systems, it is linked to tooth discolouration. The aim of this study is to evaluate and compare the antimicrobial efficacy of a newly formulated 3C antibiotic paste (consisting of Ciprofloxacin, Clindamycin, and Cefaclor) with conventional TAP against and In this in-vitro laboratory study, pure culture of and will be grown on Sabouraud's dextrose agar (SD) agar and Brain heart infusion agar (BHI) agar respectively and will be suspended for 24 hours at 37°C. For comparison of antimicrobial efficacy, the zone of inhibition will be for the 3C antibiotic paste against conventional TAP (control) for and , and will be determined using digital calliper in millimetre after every 24 and 72 hours. We hypothesise that the newly formulated 3C paste will have better antimicrobial efficacy when compared with conventional TAP. It is expected that a newly formulated 3C paste will prove to be the successful as root canal filling material for primary teeth.
乳牙根管感染本质上是多微生物感染。大多数耐药微生物，如 在根尖区根管的慢性感染中存活，并且难以被各种根管冲洗剂清除。 由于其侵入牙本质小管的能力，已在乳牙根管的慢性感染中被发现。导致牙髓感染的多种细菌菌株使得用单一有效的抗生素清洁根管变得困难。因此，先前已引入三联抗生素糊剂（TAP），其由环丙沙星、甲硝唑和米诺环素组成。尽管这种混合物有助于牙髓再生并已用于根管系统消毒，但它与牙齿变色有关。本研究的目的是评估并比较新配制的3C抗生素糊剂（由环丙沙星、克林霉素和头孢克洛组成）与传统TAP对 和 的抗菌效果。在这项体外实验室研究中， 和 的纯培养物将分别在沙氏葡萄糖琼脂（SD）琼脂和脑心浸液琼脂（BHI）琼脂上生长，并在37°C下悬浮24小时。为了比较抗菌效果，将测定3C抗生素糊剂对传统TAP（对照）针对 和 的抑菌圈，并且在每24小时和72小时后使用数字卡尺以毫米为单位进行测定。我们假设与传统TAP相比，新配制的3C糊剂将具有更好的抗菌效果。预计新配制的3C糊剂将被证明是成功的乳牙根管充填材料。
