Belanche Alejandro, Bannink André, Dijkstra Jan, Durmic Zoey, Garcia Florencia, Santos Fernanda G, Huws Sharon, Jeyanathan Jeyamalar, Lund Peter, Mackie Roderick I, McAllister Tim A, Morgavi Diego P, Muetzel Stefan, Pitta Dipti W, Yáñez-Ruiz David R, Ungerfeld Emilio M
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Wageningen Livestock Research, Wageningen University & Research, 6700 AH Wageningen, the Netherlands.
J Dairy Sci. 2025 Jan;108(1):375-394. doi: 10.3168/jds.2024-25046.
This publication aims to provide guidelines of the knowledge required and the potential research to be conducted in order to understand the mode of action of antimethanogenic feed additives (AMFA). In the first part of the paper, we classify AMFA into 4 categories according to their mode of action: (1) lowering dihydrogen (H) production; (2) inhibiting methanogens; (3) promoting alternative H-incorporating pathways; and (4) oxidizing methane (CH). The second part of the paper presents questions that guide the research to identify the mode of action of an AMFA on the rumen CH production from 5 different perspectives: (1) microbiology; (2) cell and molecular biochemistry; (3) microbial ecology; (4) animal metabolism; and (5) cross-cutting aspects. Recommendations are provided to address various research questions within each perspective, along with examples of how aspects of the mode of action of AMFA have been elucidated before. In summary, this paper offers timely and comprehensive guidelines to better understand and reveal the mode of action of current and emerging AMFA.
本出版物旨在提供所需知识及潜在研究的指导方针，以便了解抗产甲烷饲料添加剂（AMFA）的作用模式。在本文的第一部分，我们根据其作用模式将AMFA分为4类：（1）降低氢气（H）的产生；（2）抑制产甲烷菌；（3）促进替代性氢掺入途径；（4）氧化甲烷（CH）。本文的第二部分提出了一些问题，这些问题从5个不同角度指导研究以确定AMFA对瘤胃CH产生的作用模式：（1）微生物学；（2）细胞与分子生物化学；（3）微生物生态学；（4）动物代谢；（5）交叉领域。针对每个角度内的各种研究问题提供了建议，并举例说明了之前如何阐明AMFA作用模式的各个方面。总之，本文提供了及时且全面的指导方针，以更好地理解和揭示当前及新兴AMFA的作用模式。
