Ma Xiaoxuan, Zhang Zhaowei, Deng Ruoqiong, Liu Nian, Jiang Hui, Kang Zhensheng, Liu Jie
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
J Agric Food Chem. 2025 Jan 8;73(1):380-392. doi: 10.1021/acs.jafc.4c10393. Epub 2024 Dec 26.
f. sp. () secretes a plethora of cell wall-degrading enzymes (CWDEs) to facilitate fungal invasion during infection. However, the functions and molecular mechanisms of the CWDEs from remain unclear. In this study, we identified a secreted xylanase, named PstXyn1, with the GH10 domain. was significantly up-regulated at the early infection stage of The signal peptide of PstXyn1 was confirmed to be functional. The purified PstXyn1 showed detectable xylanase activity. In addition, we found that -silenced wheat plants exhibited broad-spectrum resistance against multiple pathotypes. Colloidal gold labeling and transcriptome sequencing analyses revealed that contributed to xylan degradation in host cell walls and suppressed the expression of defense-related genes. Conclusively, our results indicate that PstXyn1 is secreted as an important virulence factor to overcome host cell wall barriers and compromise immune responses for fungal invasion, providing potential targets for improving wheat resistance to stripe rust.
条锈菌（Puccinia striiformis f. sp. tritici）在侵染过程中分泌大量细胞壁降解酶（CWDEs）以促进真菌入侵。然而，条锈菌中CWDEs的功能和分子机制仍不清楚。在本研究中，我们鉴定出一种具有GH10结构域的分泌型木聚糖酶，命名为PstXyn1。PstXyn1在条锈菌侵染早期显著上调。PstXyn1的信号肽被证实具有功能。纯化后的PstXyn1表现出可检测到的木聚糖酶活性。此外，我们发现PstXyn1基因沉默的小麦植株对多种条锈菌致病型表现出广谱抗性。胶体金标记和转录组测序分析表明，PstXyn1有助于宿主细胞壁中的木聚糖降解，并抑制防御相关基因的表达。总之，我们的结果表明，PstXyn1作为一种重要的致病因子被分泌出来，以克服宿主细胞壁障碍并损害免疫反应以利于真菌入侵，为提高小麦对条锈病的抗性提供了潜在靶点。
