Senescent myoblasts exhibit an altered exometabolome that is linked to senescence-associated secretory phenotype signaling.

Cellular senescence has been implicated in the aging-related dysfunction of satellite cells, the resident muscle stem cell population primarily responsible for the repair of muscle fibers. Despite being in a state of permanent cell cycle arrest, these cells remain metabolically active and release an abundance of factors that can have detrimental effects on the cellular microenvironment. This phenomenon is known as the senescence-associated secretory phenotype (SASP), and its metabolic profile is poorly characterized in senescent muscle. In the present investigation, we examined the intracellular and extracellular metabolome of CC myoblasts using a bleomycin (BLEO)-mediated model of DNA damage-induced senescence. We also evaluated the relationship between the senescent metabolic phenotype and SASP signaling through molecular and network-based analyses. Senescent myoblasts exhibited a significantly altered extracellular metabolome (i.e., exometabolome), including increased secretion of several aging-associated metabolites. Four of these metabolites-trimethylamine--oxide (TMAO), xanthine, choline, and oleic acid-were selected for individual dose-response experiments to determine whether they could drive the senescence phenotype. Although most of the tested metabolites did not independently alter senescence markers, oleic acid treatment of healthy myoblasts significantly upregulated the SASP genes , , and ( < 0.05). A gene-metabolite interaction network further revealed that oleic acid was one of the most interconnected metabolites to key senescence-associated genes. Notably, oleic acid interacted with several prominent SASP genes, suggesting a potential epigenetic effect between this monounsaturated fatty acid and SASP regulation. In summary, the exometabolome, particularly oleic acid, is implicated in SASP signaling within senescent myoblasts. Cellular senescence and its accompanying secretory phenotype [i.e., the senescence-associated secretory phenotype (SASP)] have been linked to the aging-associated dysfunction of skeletal muscle, yet little is known about this phenomenon in satellite cells. We report that senescent myoblasts experience a significantly altered extracellular metabolome primarily characterized by the substantial release of nonesterified fatty acids. Targeted evaluation of several extracellular senescence-associated metabolites reveals a potential epigenetic role for long-chain fatty acids, particularly oleic acid, in regulating SASP-related gene expression.