Egli Patricia, Boone Lindsey, Huber Laura, Higgins Courtney, Gaonkar Pankaj P, Arrington Justine, Naskou Maria C, Peroni John, Gordon Julie, Lascola Kara M
Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
Front Vet Sci. 2024 Dec 12;11:1488942. doi: 10.3389/fvets.2024.1488942. eCollection 2024.
Platelet lysate (PL) demonstrates antimicrobial and anti-inflammatory properties offering potential for treatment of bacterial pneumonia in horses. It remains unknown whether nebulization is suitable for PL administration in horses. This pilot study characterized particle size and flow rate of pooled equine PL (single preparation) nebulized using an equine-specific nebulizer (Flexivent).
Protein composition and antimicrobial activity were compared before and after nebulization. Protein composition was evaluated according to growth factor, antimicrobial peptide and cytokine concentrations and proteomic analysis. To evaluate antimicrobial activity, bacterial growth inhibition [maximum growth (μmax); carrying capacity (K)] were determined for subsp and (WT and MDR) using pre- and post-nebulized PL concentrations of 50%.
Flow rate and median particle size were 0.8 ml/min and 4.991 μm with 52% of particles ≤ 5 μm. Differences in PL protein composition were detected with nebulization. For and , nebulization did not alter effect of PL on growth parameters. PL treatments decreased K for ( = 0.009) compared to BHI. For K was increased post- vs. pre-nebulization (WT and MDR) and μmax increased pre- vs, post-nebulization (MDR). PL treatments increased K and μmax for MDR and μmax for WT compared to BHI ( ≤ 0.05).
Nebulization of PL is technically feasible. The results of this study support further investigation to better characterize the effect of nebulization on PL and its suitability for nebulization in horses.
血小板裂解物（PL）具有抗菌和抗炎特性，为治疗马的细菌性肺炎提供了潜力。雾化是否适用于马的PL给药尚不清楚。这项初步研究对使用马专用雾化器（Flexivent）雾化的马源混合PL（单一制剂）的粒径和流速进行了表征。
比较雾化前后的蛋白质组成和抗菌活性。根据生长因子、抗菌肽和细胞因子浓度以及蛋白质组学分析评估蛋白质组成。为了评估抗菌活性，使用雾化前和雾化后50%的PL浓度测定了亚种以及（野生型和多重耐药型）的细菌生长抑制[最大生长量（μmax）；承载能力（K）]。
流速和中位粒径分别为0.8 ml/min和4.991μm，52%的颗粒≤5μm。雾化后检测到PL蛋白质组成存在差异。对于和，雾化未改变PL对生长参数的影响。与脑心浸液（BHI）相比，PL处理降低了的K（P = 0.009）。对于，雾化后K增加（野生型和多重耐药型），雾化前μmax增加（多重耐药型）。与BHI相比，PL处理增加了多重耐药型的K和μmax以及野生型的μmax（P≤0.05）。
PL的雾化在技术上是可行的。本研究结果支持进一步研究，以更好地表征雾化对PL的影响及其在马中雾化的适用性。
