Investigating the neuroprotective properties of extract against MPP-induced apoptosis in SH-SY5Y human neuroblastoma cells.

This study aims to assess the neuroprotective effects of the methanolic extract of against oxidative stress and cell death induced by neurotoxins MPP  in SH-SY5Y cells. Briefly, the methanolic extract of decreased the cytotoxicity of MPP  in SH-SY5Y cells. Treatment with extract at a concentration of 400 µg/ml resulted in a notable decrease in cell death, particularly in MPP  -induced cells. Flow cytometry analysis with annexin V/PI staining reveals apoptosis and necrosis in SH-SY5Y cell lines upon exposure to 1 mM of MPP  . However, 100-400 µg/ml concentrations of extract effectively decrease apoptosis in SH-SY5Y cells. Furthermore, inhibits caspase-3 activity, effectively shielding neuronal cells against MPP  -induced cell death. Mitochondrial membrane potential (MMP) assay using a JC-1 fluorescent probe indicates that the methanolic extract of exhibits protective effects against MPP  -induced cell death and maintains mitochondrial membrane potential. Our results conclude that exposing SH-SY5Y cells to a methanolic extract of could potentially increase the likelihood of inhibiting the cascade mechanism, stopping MPP-induced apoptosis, and preventing the rupture of the mitochondrial membrane. However, the lack of low solubility and poor bioavailability reduce the therapeutic efficacy of . Liposome-based drug delivery systems can improve the bioavailability and stability of bioactive compounds, enhancing their therapeutic potential. Hence, may hold promise as an innovative treatment for neurological ailments.