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利用藻类提取物绿色合成的氧化锌纳米颗粒的抗血管生成和抗凋亡作用

Antiangiogenic and antiapoptotic effects of green-synthesized zinc oxide nanoparticles using algae extraction.

作者信息

Sanaeimehr Zahra, Javadi Iraj, Namvar Farideh

机构信息

Department of Toxicology, Faculty of Pharmacy, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan Iran.

2Departments of Medicine & AMP; Applied Biology Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

出版信息

Cancer Nanotechnol. 2018;9(1):3. doi: 10.1186/s12645-018-0037-5. Epub 2018 Apr 2.

DOI:10.1186/s12645-018-0037-5
PMID:29628994
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5879045/
Abstract

BACKGROUND

Algae are one of the natural materials used to green synthesis of nanoparticles. This method leads to minimize the toxicity of the chemical materials used to nanoparticle synthesis.

METHODS

In this study, zinc oxide nanoparticles (ZnO NPs) synthesized by algae extraction used to evaluate its cytotoxicity and apoptotic properties on human liver cancer cell line (HepG2).

RESULTS

Trypan blue assay results demonstrate a concentration-dependent decrease in cell viability and MTT assay shows increased growth inhibition in time and dose-dependent manner. In addition, CAM assay confirmed the ability of ZnO NPs to inhibit angiogenesis, but chick morphology (both the CR and weight) was not changed. Apoptotic tests (annexin V/PI and AO/PI) show that green-synthesized ZnO NPs induce apoptosis in all three time points (24, 48 and 72h).

CONCLUSIONS

Our results confirm the beneficial cytotoxic effects of green-synthesized ZnO NPs on Human liver cancer cell. This nanoparticle decreased angiogenesis and induces apoptosis, so we conclude that these nanoparticles can be used as a supplemental drug in cancer treatments.

摘要

背景

藻类是用于纳米颗粒绿色合成的天然材料之一。这种方法可将用于纳米颗粒合成的化学材料的毒性降至最低。

方法

在本研究中，通过藻类提取物合成的氧化锌纳米颗粒（ZnO NPs）用于评估其对人肝癌细胞系（HepG2）的细胞毒性和凋亡特性。

结果

台盼蓝测定结果表明细胞活力呈浓度依赖性降低，MTT测定显示生长抑制呈时间和剂量依赖性增加。此外，鸡胚绒毛尿囊膜（CAM）试验证实了ZnO NPs抑制血管生成的能力，但鸡胚形态（包括绒毛尿囊膜和重量）未发生变化。凋亡试验（膜联蛋白V/碘化丙啶和吖啶橙/碘化丙啶）表明，绿色合成的ZnO NPs在所有三个时间点（24、48和72小时）均诱导细胞凋亡。

结论

我们的结果证实了绿色合成的ZnO NPs对人肝癌细胞具有有益的细胞毒性作用。这种纳米颗粒可减少血管生成并诱导细胞凋亡，因此我们得出结论，这些纳米颗粒可作为癌症治疗的辅助药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/58d9d635f86e/12645_2018_37_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/7b86964661da/12645_2018_37_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/d60abf4e2d00/12645_2018_37_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/910162c3e083/12645_2018_37_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/7f5cce3bffbd/12645_2018_37_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/72fb4968f44d/12645_2018_37_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/5672cceab9b4/12645_2018_37_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/5acd97c73a44/12645_2018_37_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/58d9d635f86e/12645_2018_37_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/7b86964661da/12645_2018_37_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/d60abf4e2d00/12645_2018_37_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/910162c3e083/12645_2018_37_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/7f5cce3bffbd/12645_2018_37_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/72fb4968f44d/12645_2018_37_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/5672cceab9b4/12645_2018_37_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/5acd97c73a44/12645_2018_37_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/838f/5879045/58d9d635f86e/12645_2018_37_Fig8_HTML.jpg

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