Dalvand Leila Firouzi, Hosseini Farzaneh, Dehaghi Shahram Moradi, Torbati Elham Siasi
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Iran J Biotechnol. 2018 Dec 12;16(4):e2102. doi: 10.21859/ijb.2102. eCollection 2018 Dec.
Based on the increase in antibiotic-resistant pathogens, it is necessary to have various effective compounds, so as to prevent its proliferation of these pathogens. For this purpose, nano-materials such as bismuth oxide nanoparticles can be used.
The aim of this study was to produce bismuth oxide nanoparticles by PTCC1320 and to determine the antimicrobial effects on methicillin-resistant species compared with some antibiotics.
In this study, 200 bacterial samples were collected from hospitalized patients with burn infections from the Burn Rescue Hospital, Tehran. Thereafter, 65 strains of methicillin-resistant were identified by their phenotype and genotype. A total of 92% of identified strains with the highest resistance to antibiotics were isolated. Bismuth oxide nanoparticles were synthesized by PTCC1320. FTIR spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to analyze the extracellularly produced nanoparticles. Finally, the antibacterial properties of nanoparticles produced on the biofilm of some pathogens were examined.
In the present study, cube-shaped bismuth oxide nanoparticles were formed in the size range of 29-62 nm. They were found to have antimicrobial activity on 16% of the isolated strains. The FTIR results showed the vibrational frequencies of bismuth oxide at 583, 680, 737, and 1630 nm. The XRD results also confirmed the structure of nanoparticles. Compared with antibiotics such as Ciprofloxacin, bismuth oxide nanoparticles had less affectivity on this resistant hospital pathogen. Increasing the concentration of bismuth oxide nanoparticles, increased its antimicrobial effect and decreased bacterial growth rate.
Compared with heavy metals, bismuth nanoparticles have very low antibacterial effects. Considering this feature, the use of less antibiotics can be achieved with bismuth nanoparticles in the treatment of infections, thereby reducing antibiotic resistance.
基于对抗生素耐药病原体的增加，有必要拥有各种有效的化合物，以防止这些病原体的扩散。为此，可以使用氧化铋纳米颗粒等纳米材料。
本研究的目的是通过PTCC1320制备氧化铋纳米颗粒，并与一些抗生素相比，确定其对耐甲氧西林菌株的抗菌作用。
在本研究中，从德黑兰烧伤急救医院的烧伤感染住院患者中收集了200份细菌样本。此后，通过表型和基因型鉴定出65株耐甲氧西林菌株。分离出了总共92%对抗生素耐药性最高的菌株。通过PTCC1320合成氧化铋纳米颗粒。使用傅里叶变换红外光谱（FTIR）、X射线衍射和扫描电子显微镜（SEM）分析细胞外产生的纳米颗粒。最后，检测了所产生的纳米颗粒对一些病原体生物膜的抗菌性能。
在本研究中，形成了尺寸范围为29-62nm的立方体形氧化铋纳米颗粒。发现它们对16%的分离菌株具有抗菌活性。FTIR结果显示氧化铋在583、680、737和1630nm处的振动频率。XRD结果也证实了纳米颗粒的结构。与环丙沙星等抗生素相比，氧化铋纳米颗粒对这种耐药医院病原体的影响较小。增加氧化铋纳米颗粒的浓度，其抗菌作用增强，细菌生长速率降低。
与重金属相比，铋纳米颗粒的抗菌作用非常低。考虑到这一特性，在感染治疗中使用铋纳米颗粒可以减少抗生素的使用，从而降低抗生素耐药性。
