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提取物在脂多糖刺激的RAW 264.7细胞中的抗炎作用。

Anti-Inflammatory Effects of Extract in -Stimulated RAW 264.7 Cells.

作者信息

Kim Min-Jeong, Kim Hyun-Jin

机构信息

Department of Convergence Technology for Food Industry, Wonkwang University, Iksan 54538, Republic of Korea.

Institute of Biomaterial • Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.

出版信息

Curr Issues Mol Biol. 2024 Nov 24;46(12):13405-13417. doi: 10.3390/cimb46120799.

DOI:10.3390/cimb46120799
PMID:39727927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11727614/
Abstract

has been used both as a food and in traditional medicine. However, its anti-inflammatory effects in periodontal diseases have not been studied. We examined the anti-inflammatory properties of extract in RAW 264.7 cells stimulated by . The cytotoxicity of extract was evaluated using the MTS assay. Its effect on NO production was then measured using the NO assay. The mRNA expression of inducible nitric oxide synthase (iNOS) and the pro-inflammatory cytokines IL-1β and IL-6 were assessed using quantitative real-time PCR (qRT-PCR). Western blotting was performed to investigate the expression of regulatory proteins involved in the NF-κB and MAPK signaling pathways. extract significantly inhibited NO production without cytotoxicity in RAW 264.7 cells. Following stimulation, treatment with the extract decreased iNOS mRNA expression and protein levels, which are responsible for NO production. The extract also suppressed the mRNA expression of pro-inflammatory cytokines. Additionally, extract inhibited NF-κB activation by regulating signaling molecules such as IKK and IκBα, while also preventing the phosphorylation of MAPK, including ERK, p38, and JNK, showing anti-inflammatory potential. Therefore, it may be a promising natural candidate for the development of new preventive and therapeutic strategies for periodontitis.

摘要

它既被用作食物，也被用于传统医学。然而，其在牙周疾病中的抗炎作用尚未得到研究。我们检测了提取物在由 刺激的RAW 264.7细胞中的抗炎特性。使用MTS法评估提取物的细胞毒性。然后使用NO检测法测量其对NO产生的影响。使用定量实时PCR（qRT-PCR）评估诱导型一氧化氮合酶（iNOS）以及促炎细胞因子IL-1β和IL-6的mRNA表达。进行蛋白质免疫印迹法以研究参与NF-κB和MAPK信号通路的调节蛋白的表达。提取物在RAW 264.7细胞中显著抑制NO产生且无细胞毒性。在 刺激后，用提取物处理可降低负责NO产生的iNOS mRNA表达和蛋白水平。该提取物还抑制促炎细胞因子的mRNA表达。此外，提取物通过调节诸如IKK和IκBα等信号分子来抑制NF-κB激活，同时还阻止包括ERK、p38和JNK在内的MAPK的磷酸化，显示出抗炎潜力。因此，它可能是开发牙周炎新预防和治疗策略的有前景的天然候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/ff75b7be017e/cimb-46-00799-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/7cac598e243c/cimb-46-00799-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/d49401cc4efc/cimb-46-00799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/7dbc4d316679/cimb-46-00799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/c88e9185a621/cimb-46-00799-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/e05acf1cbd34/cimb-46-00799-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/99e73cbe1b03/cimb-46-00799-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/ff75b7be017e/cimb-46-00799-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/7cac598e243c/cimb-46-00799-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/2cce7b447eec/cimb-46-00799-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/d49401cc4efc/cimb-46-00799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/7dbc4d316679/cimb-46-00799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/c88e9185a621/cimb-46-00799-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/e05acf1cbd34/cimb-46-00799-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/99e73cbe1b03/cimb-46-00799-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e3e/11727614/ff75b7be017e/cimb-46-00799-g008.jpg

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本文引用的文献

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Role of Interleukin-17A in the Pathomechanisms of Periodontitis and Related Systemic Chronic Inflammatory Diseases.白细胞介素-17A 在牙周炎及相关系统性慢性炎症性疾病发病机制中的作用。
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