Department of Smart Foods and Drugs, Inje University, Gimhae 621-749, Republic of Korea.
J Ethnopharmacol. 2012 Mar 27;140(2):271-6. doi: 10.1016/j.jep.2012.01.016. Epub 2012 Jan 20.
Orostachys japonicus A. Berger (O. japonicus) is known to reduce the risk of many diseases.
We investigated the anti-inflammatory effects of the dichloromethane (DCM) fraction from O. japonicus (OJD) in LPS-stimulated RAW 264.7 cells.
NO was measured using the Griess method. Key pro-inflammatory cytokines and mediators including IL-1β, TLR4, iNOS, and COX-2; 2 important pro-inflammatory transcription factors, NF-κB p65 and IκBα; and MAPKs such as ERK1/2, JNK, and p38 were analyzed by Western blotting.
OJD significantly inhibited NO production, IL-1β, TLR4, iNOS, and COX-2 expression in LPS-stimulated cells. Additionally, it inhibited LPS-induced NF-κB p65 activation via inhibition of IκBα phosphorylation. Furthermore, phosphorylation of p38 and JNK was suppressed by OJD in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells.
Our data suggest that OJD inhibits the inflammatory response via suppression of NF-κB activation and MAPK signaling.
东方狗娃花（Orostachys japonicus A. Berger）被认为可以降低许多疾病的风险。
我们研究了东方狗娃花二氯甲烷（DCM）馏分（OJD）对 LPS 刺激的 RAW 264.7 细胞的抗炎作用。
采用 Griess 法测定 NO。通过 Western blot 分析关键促炎细胞因子和介质，包括 IL-1β、TLR4、iNOS 和 COX-2；2 个重要的促炎转录因子，NF-κB p65 和 IκBα；以及 MAPKs，如 ERK1/2、JNK 和 p38。
OJD 显著抑制 LPS 刺激细胞中 NO 的产生、IL-1β、TLR4、iNOS 和 COX-2 的表达。此外，它通过抑制 IκBα磷酸化来抑制 LPS 诱导的 NF-κB p65 激活。此外，OJD 以剂量依赖的方式抑制 LPS 刺激的 RAW 264.7 细胞中 p38 和 JNK 的磷酸化。
我们的数据表明，OJD 通过抑制 NF-κB 激活和 MAPK 信号通路来抑制炎症反应。
