Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, Seoul 130-872, Republic of Korea.
Evid Based Complement Alternat Med. 2015;2015:682019. doi: 10.1155/2015/682019. Epub 2015 Feb 24.
Orostachys japonicus is traditionally used as an inflammatory agent. In this report, we investigated the effects of O. japonicus extract on the expression of genes encoding pathogen-recognition receptors (TLR2, TLR4, NOD1, and NOD2) and proinflammatory factors (iNOS, COX-2, and cytokines) in LPS-stimulated PMA-differentiated THP-1 cells and the NF-κB and MAPK pathways. O. japonicus induced toxicity at high concentrations but had no effect at concentrations lower than 25 μg/mL. O. japonicus inhibited LPS-induced TLR4 and NOD2 mRNA levels, suppressed LPS-induced iNOS and COX-2 transcription and translocation, and downregulated LPS-induced proinflammatory cytokine (IL-1β, IL-6, IL-8, and TNF-α) mRNA levels. In addition, O. japonicus inhibited LPS-induced NF-κB activation and IκBα degradation and suppressed LPS-induced JNK, p38 MAPK, and ERK phosphorylation. Overall, our results demonstrate that the anti-inflammatory effects of O. japonicus are mediated by suppression of NF-κB and MAPK signaling, resulting in reduced TLR4, NOD2, iNOS, and COX-2 expression and inhibition of inflammatory cytokine expression.
瓦松被传统用作一种炎症介质。在本报告中，我们研究了瓦松提取物对 LPS 刺激的 PMA 分化 THP-1 细胞中编码病原体识别受体（TLR2、TLR4、NOD1 和 NOD2）和促炎因子（iNOS、COX-2 和细胞因子）的基因表达以及 NF-κB 和 MAPK 通路的影响。瓦松在高浓度下具有细胞毒性，但在低于 25μg/mL 的浓度下没有影响。瓦松抑制 LPS 诱导的 TLR4 和 NOD2 mRNA 水平，抑制 LPS 诱导的 iNOS 和 COX-2 转录和易位，并下调 LPS 诱导的促炎细胞因子（IL-1β、IL-6、IL-8 和 TNF-α）mRNA 水平。此外，瓦松抑制 LPS 诱导的 NF-κB 激活和 IκBα 降解，并抑制 LPS 诱导的 JNK、p38 MAPK 和 ERK 磷酸化。总的来说，我们的结果表明，瓦松的抗炎作用是通过抑制 NF-κB 和 MAPK 信号转导介导的，从而减少 TLR4、NOD2、iNOS 和 COX-2 的表达，并抑制炎症细胞因子的表达。
