Aldogan Ebru Haciosmanoglu, Başaran Deniz, Öner Bilgin, Günçer Başak
Department of Biophysics, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, 34098 Fatih, Istanbul, Turkey.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University, 34093 Fatih, Istanbul, Turkey.
Curr Issues Mol Biol. 2024 Dec 17;46(12):14215-14225. doi: 10.3390/cimb46120851.
Dental bone formation involves various cellular and molecular mechanisms, and phytoestrogens such as formononetin (FORM) are promising because of their estrogenic, anti-inflammatory, and antioxidant effects. This study investigated the effect of FORM on osteoblast proliferation, differentiation, and mineralization in combination with spongiosa granulates (BO) in vitro.
Human fetal osteoblast cells (hFOB1.19) were treated with increasing concentrations of FORM (1, 10, and 100 µg/mL), BO, or their combination. Cell proliferation was assessed using a MTT assay. Alkaline phosphatase (ALP) activity, intracellular Ca, and Pi levels were measured using ELISA. Vascular endothelial growth factor (VEGF) and osteocalcin expression levels were analyzed by western blotting.
Cell proliferation increased with FORM, with or without BO, after 6 days ( < 0.001). FORM and BO had a synergistic effect on ALP activity ( < 0.001). Intracellular Ca and Pi levels were highest in the BO-FORM group, suggesting superior mineralization ( < 0.05). VEGF and osteocalcin expression was significantly upregulated with FORM, alone and with BO ( < 0.05), indicating improved angiogenesis and bone maturation over 9 days.
FORM enhances osteoblast proliferation, differentiation, and mineralization potential, particularly in BO spongiosa granulates. These data support the in vitro potential of formononetin-phytoestrogen in promoting osteoblast differentiation and mineralization potential with BO. These findings suggest that FORM, combined with BO, could improve bone augmentation in clinical applications such as maxillofacial surgery. FORM shows valuable potential for clinical applications, such as maxillofacial surgery, by promoting faster and more effective healing.
牙骨质形成涉及多种细胞和分子机制，而诸如芒柄花黄素（FORM）等植物雌激素因其雌激素样、抗炎和抗氧化作用而颇具前景。本研究在体外研究了FORM与松质颗粒（BO）联合使用对成骨细胞增殖、分化和矿化的影响。
用递增浓度的FORM（1、10和100µg/mL）、BO或它们的组合处理人胎儿成骨细胞（hFOB1.19）。使用MTT法评估细胞增殖。使用酶联免疫吸附测定法测量碱性磷酸酶（ALP）活性、细胞内钙和磷水平。通过蛋白质免疫印迹法分析血管内皮生长因子（VEGF）和骨钙素的表达水平。
6天后，无论有无BO，FORM均可使细胞增殖增加（P<0.001）。FORM和BO对ALP活性具有协同作用（P<0.001）。BO-FORM组的细胞内钙和磷水平最高，表明矿化效果更佳（P<0.05）。单独使用FORM以及与BO联合使用时，VEGF和骨钙素的表达均显著上调（P<0.05），表明在9天内血管生成和骨成熟得到改善。
FORM可增强成骨细胞的增殖、分化和矿化潜能，尤其是在BO松质颗粒存在的情况下。这些数据支持了芒柄花黄素-植物雌激素在体外与BO联合促进成骨细胞分化和矿化潜能的可能性。这些发现表明，FORM与BO联合使用可改善诸如颌面外科等临床应用中的骨增量。FORM通过促进更快、更有效的愈合，在诸如颌面外科等临床应用中显示出有价值的潜力。
